LOCUS       sequence-278963-        2447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0540
FEATURES             Location/Qualifiers
     source          1..2447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctcacag gccgaaaagg acttttcggc agcgcgcggc tgattccagc
       61 gacagcgatg gcgccgagga gtcgcctgct gagcctgggg cgccgaggga acttccggtc
      121 ccgggttctg cggaggaaga gccgccctct ggaggaggcc gcgcgcaggt ggcgggactg
      181 ccccaccggg ttcggggccc tcgtggccgg ggccgggtct gggcgagctc ccggcgtgcc
      241 accaaagcgg ctccccgcgc ggacgaaggc tcagaatcca gaacccttga tgtgtccaca
      301 gatgaagagg ataaaataca tcactcctca gaaagtaagg atgatcaggg tttgtcttct
      361 gacagttcta gctctcttgg agaaaaagaa ctttcatcaa cagttaagat cccagatgca
      421 gcttttattc aggcagcccg cagaaaacgt gaattggcca gggcccaaga tgactatatt
      481 tctttggatg tacaacatac ctcctccatc tctggtatga agagagagag cgaagatgac
      541 cctgagagtg agcctgatga ccatgaaaag agaataccat ttactctaag acctcaaaca
      601 cttagacaaa ggatggctga ggaatcaata agcagaaatg aagaaacaag tgaagaaagt
      661 caggaagatg aaaagcaaga tacttgggaa caacagcaaa tgaggaaagc agttaaaatc
      721 atagaggaaa gagacataga tctttcctgt ggcaatggat cttcaaaagt gaagaaattt
      781 gatacttcca tttcatttcc gccagtaaat ttagaaatta taaagaagca attaaatact
      841 agattaacat tactacagga aactcaccgc tcacacctga gggagtatga aaaatacgta
      901 caagatgtca aaagctcaaa gagtaccatc cagaacctag agagttcatc aaatcaagct
      961 ctaaattgta aattctataa aagcatgaaa atttatgtgg aaaatttaat tgactgcctt
     1021 aatgaaaaga ttatcaacat ccaagaaata gaatcatcca tgcatgcact ccttttaaaa
     1081 caagctatga cctttatgaa acgcaggcaa gatgaattaa aacatgaatc aacgtattta
     1141 caacagttat cacgcaaaga tgagacatcc acaagtggaa acttctcagt agatgaaaaa
     1201 actcagtgga ttttagaaga gattgaatct cgaaggacaa aaagaagaca agcaagggtg
     1261 ctttctggga attgtaacca tcaggaagga acatctagtg atgatgaact gccttcagca
     1321 gagatgattg acttccaaaa aagccaaggt gacattttac agaaacagaa gaaagttttt
     1381 gaagaagtgc aagatgattt ttgtaacatc cagaatattt tgttgaaatt tcagcaatgg
     1441 cgagaaaagt ttcctgactc ctattatgaa gctttcatta gtttatgcat accaaagctt
     1501 ttaaatcccc taatacgagt tcagttgatt gattggaatc ctcttaagtt ggaatccaca
     1561 ggtttaaaag agatgccatg gttcaaatct gtagaagaat ttatggatag cagtgtggaa
     1621 gattcaaaga aggaaagtag ttcagataaa aaagtcttgt ctgcaatcat caacaaaaca
     1681 attattcccc gacttacaga ctttgtagaa ttcctttggg atcctttgtc aacctcacag
     1741 acaacaagtt taataacaca ttgcagagtg attcttgaag aacattccac ttgtgaaaat
     1801 gaagttagta aaagcagaca ggatttactt aaatccattg tttcaagaat gaaaaaggca
     1861 gtagaagatg atgtttttat tcctctgtat ccaaagagtg ctgtagaaaa caaaacatca
     1921 cctcattcaa agttccaaga aagacagttc tggtcaggcc taaagctctt ccgcaatatt
     1981 cttctttgga atggactcct tacagatgac accttgcaag aactaggact agggaagctg
     2041 ctaaatcgtt accttattat agcacttctc aatgccacac ctgggccaga tgtggttaaa
     2101 aagtgcaacc aggtagcagc atgtctacca gaaaaatggt ttgaaaattc tgccatgagg
     2161 acatctattc cacagctaga aaacttcatt cagtttttat tgcagtctgc acataaatta
     2221 tctagaagtg aattcaggga tgaagtcgaa gaaataattc ttattttggt gaaaataaaa
     2281 gctttgaatc aagcagaatc cttcatagga gagcatcacc tagaccatct taaatcacta
     2341 attaaagaag attagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2401 tatatatctt gtggaaagga cgatgtaaga gcgggtagag cccagat
//
