LOCUS       sequence-278972-        2588 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0625
FEATURES             Location/Qualifiers
     source          1..2588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggctga agaggcggat gtggatatcg aaggggacgt ggtagcggcg
       61 gcgggggcac agccaggaag tggtgaaaat acagcatcag ttttacaaaa agatcactat
      121 cttgattcat cttggagaac agagaatggc cttattcctt ggaccttgga taacaccatc
      181 agtgaagaga acagagctgt tattgagaaa atgttgttgg aagaagaata ttatttatct
      241 aaaaaatcac aaccggaaaa agtctggctt gatcaaaagg aagatgataa aaaatacatg
      301 aagagtctgc agaaaacagc aaaaatcatg gtacactctc ctacaaaacc agccagttac
      361 tcagtaaagt ggacgataga agaaaaagag ctgtttgaac aagggctggc taaatttggc
      421 cgaagatgga ccaaaatttc aaagctaatt ggaagccgca ctgttttaca agtgaagagt
      481 tatgcaagac agtattttaa aaataaggtc aaatgcggtc tggataaaga aacaccaaat
      541 cagaagaccg gccataatct tcaagttaaa aatgaagata aagggacaaa ggcatggaca
      601 ccatcatgtt taaggggacg tgctgatccc aacttgaatg ctgtaaaaat tgaaaagtta
      661 tctgatgatg aagaagtaga catcacagat gaggtggacg agttgtcttc tcaaacaccc
      721 cagaagaatt ctagcagtga tctcttgtta gactttccta atagtaaaat gcatgaaacc
      781 aatcaaggag aattcattac ttctgacagc caggaagctc tcttttctaa gtcttccagg
      841 ggctgtcttc aaaatgaaaa gcaagatgaa acactttcaa gctcagaaat tacactgtgg
      901 actgagaaac agagcaatgg tgacaaaaaa tcaattgaat taaatgacca gaaatttaat
      961 gaattgatta aaaactgcaa caagcatgat ggaaggggaa taatagttga tgccaggcag
     1021 ttgccttctc cagagccttg tgaaattcag aaaaatttga atgataatga aatgcttttt
     1081 cattcttgcc aaatggtaga ggaaagccat gaggaagaag agcttaagcc accagaacag
     1141 gaaatagaaa tagatagaaa tatcattcaa gaagaagaaa aacaagcaat tcctgagttt
     1201 tttgaggggc gccaagctaa aacaccagaa cgctatttga aaattagaaa ttatattttg
     1261 gatcaatggg agatatgcaa accaaaatac ttaaataaga cctcagtacg tcctggcctg
     1321 aagaactgtg gagatgttaa ttgtattgga cggattcata catacctcga attgatagga
     1381 gcaatcaatt ttggatgtga acaggctgtg tataataggc cacaaacagt tgacaaagta
     1441 cgaatcagag acagaaaaga tgcagtagaa gcataccaac ttgcccagcg tctgcagtct
     1501 atgcgtacaa ggagacgtag ggtccgagac ccatggggaa actggtgtga tgcaaaggac
     1561 ttagaaggac aaacgtttga gcatctctct gctgaggagt tggcaaaaag aagagaagag
     1621 gaaaaaggca gacctgttaa atctttaaaa gtgccaagac caacaaaaag ctcgtttgat
     1681 cccttccaac tgataccttg taattttttt agtgaagaaa agcaggagcc atttcaggtg
     1741 aaagtggctt cagaagcact tttaataatg gatttgcatg ctcatgtttc tatggcagaa
     1801 gtgattggtc tgttaggagg aagatactca gaagttgata aagtagttga agtctgtgca
     1861 gcagaaccat gtaacagtct gagtacagga ctacagtgtg agatggatcc tgtatcacaa
     1921 acacaggcct cagaaacctt ggctgttaga ggcttcagtg ttattggatg gtatcattct
     1981 catcctgctt ttgatcctaa tccttcctta cgagatattg acacacaagc taaataccag
     2041 agttacttct ccagaggagg tgcaaagttc attgggatga ttgttagtcc ctataatcga
     2101 aataatccct taccatattc tcagattacc tgcctggtta taagtgagga aattagccca
     2161 gatggctctt atcgcttacc ttacaaattt gaagtacagc agatgttaga agaacctcag
     2221 tggggattag tatttgaaaa gacaagatgg ataatagaaa aatacaggct ctcccatagc
     2281 agcgtcccca tggataaaat ctttcgccgg gattctgacc tgacttgttt gcagaaactt
     2341 ttggagtgta tgaggaagac tctgagcaaa gtgaccaatt gctttatggc tgaagaattc
     2401 ttgactgaaa tagaaaattt gttcctttcc aattataaaa gcaaccaaga gaatggagta
     2461 accgaagaga actgtacaaa ggaattgtta atgtagtaat gaactagtcc gtaacttgaa
     2521 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaccggcg ttcacaggtt
     2581 cctctagg
//
