LOCUS       sequence-278981-        1421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0631
FEATURES             Location/Qualifiers
     source          1..1421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagga tgtttcctca gcggccccga gcccgcgggg ctgtgcggat
       61 ggtagggatg ccgaccctac tgaggagcag atggcagaaa cagagagaaa cgacgaggag
      121 cagttcgaat gccaggaact gctcgagtgc caggtgcagg tgggggcccc cgaggaggag
      181 gaggaggagg aggaggacgc gggcctggtg gccgaggccg aggccgtggc tgccggctgg
      241 atgctcgatt tcctctgcct ctctctttgc cgagctttcc gcgacggccg ctccgaggac
      301 ttccgcagga cccgcaacag cgcagaggct attattcatg gactatccag tctaacagct
      361 tgccagttga gaacgatata catatgtcag tttttgacaa gaattgcagc aggaaaaacc
      421 cttgatgcac agtttgaaaa tgatgaacga attacaccct tggaatcagc cctgatgatt
      481 tggggttcaa ttgaaaagga acatgacaaa cttcatgaag aaatacagaa tttaattaaa
      541 attcaggcta tagctgtttg tatggaaaat ggcaacttta aagaagcaga agaagtcttt
      601 gaaagaatat ttggtgatcc aaattctcat atgcctttca aaagcaaatt gcttatgata
      661 atctctcaga aagatacatt tcattccttt tttcaacact tcagctacaa ccacatgatg
      721 gagaaaatta agagttatgt gaattatgtg ctaagtgaaa aatcatcaac ctttctaatg
      781 aaggcagcgg caaaagtagt agaaagcaaa aggacaagaa caataacttc tcaagataaa
      841 cctagtggta atgatgttga aatggaaact gaagctaatt tggatacaag aaaaagtgtt
      901 agtgacaaac agtctgcggt aactgaatcc tcagagggta cagtatcctt attgaggtct
      961 cacaagaatc ttttcttatc taagttgcaa catggaaccc agcaacaaga ccttaataag
     1021 aaagaaagaa gagtaggaac tcctcaaagt acaaaaaaga aaaaagaaag cagaagagcc
     1081 actgaaagca gaatacctgt ttcaaagagt cagccggtaa ctcctgaaaa acatcgagct
     1141 agaaaaagac aggcatggct ttgggaagaa gacaagaatt tgagatctgg cgtgaggaaa
     1201 tatggagagg gaaactggtc taaaatactg ttgcattata aattcaacaa ccggacaagt
     1261 gtcatgttaa aagacagatg gaggaccatg aagaaactaa aactgatttc ctcagacagc
     1321 gaagactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1381 tcttgtggaa aggacgatga agactttaga ctgattatgg g
//
