LOCUS       sequence-279005-        2006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1921
FEATURES             Location/Qualifiers
     source          1..2006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagccatc tcctctgtct cccagtgggg cagcacttcc cctgccgctg
       61 tcgctggctc cgcccccact acccctgcca gcagctgcag tggtacatgt gtccttccct
      121 gaggtgacca gtgccctctt ggagtccctc aatcagcagc gtctgcaggg ccagctctgc
      181 gatgtatcta tcagagtgca gggccgggag ttccgggctc atcgggctgt cctggctgcc
      241 tcctcccctt acttccatga tcaggtccta ctcaaaggca tgacctccat ctcgctgccc
      301 agtgtcatgg acccaggcgc ctttgagact gtcctagcct ccgcttacac tggccgcctc
      361 agcatggctg ctgctgacat tgtcaacttc cttacagtgg ggtctgtgct ccaaatgtgg
      421 cacattgtgg acaagtgcac tgaactactc cgagaaggcc gggcctcagc taccaccacc
      481 atcactactg ctgcagccac ctctgtcact gtccctggtg ctggggtgcc atccgggagt
      541 gggggcactg tggcccctgc taccatgggc tctgcgcgct cccatgcctc cagccgggcc
      601 agtgagaatc aatctcccag cagcagcaac tacttcagcc ccagggagtc cactgatttc
      661 tcatcttcct cccaagaggc atttgcagct tctgcagtgg gcagtgggga gcgtcgagga
      721 ggtggccctg tattcccagc ccctgtcgtt ggcagtggag gggccacatc tggaaagctg
      781 ctgctggagg cagatgagct gtgcgatgat ggtggggatg ggaggggggc agtggttcct
      841 ggggctgggc tccggagacc cacctacaca ccccctagca tcatgccaca gaaacactgg
      901 gtatacgtga agcgaggtgg taattgccca gcgccaacac ccctggttcc ccaagaccca
      961 gatctggagg aggaagagga ggaggaagat ctggtgttga cctgtgagga tgatgaagat
     1021 gaagaactag ggggtagctc cagggttcca gtggggggag ggcctgaggc taccctcagc
     1081 ataagtgatg tccgtaccct gagtgagccc ccagacaagg gggaggagca ggtcaacttc
     1141 tgtgagtcct ccaatgactt tggcccatat gagggtgggg gtcctgtggc aggtcttgat
     1201 gactcagggg ggccaactcc ctcttcctat gccccctccc accctcctcg accgctcctt
     1261 cccttggaca tgcagggcaa ccagatcctg gtcttcccgt cgtcgtcttc atcctcatcc
     1321 tcacaggctc ctggccaacc accagggaac caagcagaac acggggcagt gaccgtgggg
     1381 ggcacgtcgg tggggagcct gggtgtgccg ggtagcgttg gtggggtccc tggagggact
     1441 ggcagtgggg acgggaataa gatctttctg tgccattgtg ggaaggcctt ctcccacaag
     1501 agcatgcggg accggcacgt gaacatgcac ctcaatctgc ggccgtttga ctgccccgtg
     1561 tgcaacaaaa agttcaagat gaagcaccat ctgactgagc acatgaagac gcacacaggt
     1621 ctcaagccct acgagtgcgg agtctgcgcc aagaagttca tgtggcgaga cagcttcatg
     1681 cgccaccgag gacactgtga gcgccggcac cgcctgggcg gggtcggggc cgtacctggg
     1741 cctgggactc ccacggggcc atccttgccg tccaagagag agtctcccgg agtgggcggg
     1801 ggcagcggcg acgaagcgag tgcggccacg cccccgtcca gcagacgtgt ctggtcccca
     1861 cccagagtcc acaaggtgga gatgggcttc ggtggaggtg gaggagcaaa ctagtaatga
     1921 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     1981 gacgacgcta tagttcaacg actctc
//
