LOCUS       sequence-279032-        1547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1957
FEATURES             Location/Qualifiers
     source          1..1547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatataaa aaactcacca tctagcctta attctccttc ctcctacaac
       61 tgcagtcaat ccatcttacc cctggagcac ggctccatat acataccttc ctcctatgta
      121 gacagccacc atgaatatcc agccatgaca ttctatagcc ctgctgtgat gaattacagc
      181 attcccagca atgtcactaa cttggaaggt gggcctggtc ggcagaccac aagcccaaat
      241 gtgttgtggc caacacctgg gcacctttct cctttagtgg tccatcgcca gttatcacat
      301 ctgtatgcgg aacctcaaaa gagtccctgg tgtgaagcaa gatcgctaga acacacctta
      361 cctgtaaaca gagagacact gaaaaggaag gttagtggga accgttgcgc cagccctgtt
      421 actggtccag gttcaaagag ggatgctcac ttctgcgctg tctgcagcga ttacgcatcg
      481 ggatatcact atggagtctg gtcgtgtgaa ggatgtaagg ccttttttaa aagaagcatt
      541 caaggacata atgattatat ttgtccagct acaaatcagt gtacaatcga taaaaaccgg
      601 cgcaagagct gccaggcctg ccgacttcgg aagtgttacg aagtgggaat ggtgaagtgt
      661 ggctcccgga gagagagatg tgggtaccgc cttgtgcgga gacagagaag tgccgacgag
      721 cagctgcact gtgccggcaa ggccaagaga agtggcggcc acgcgccccg agtgcgggag
      781 ctgctgctgg acgccctgag ccccgagcag ctagtgctca ccctcctgga ggctgagccg
      841 ccccatgtgc tgatcagccg ccccagtgcg cccttcaccg aggcctccat gatgatgtcc
      901 ctgaccaagt tggccgacaa ggagttggta cacatgatca gctgggccaa gaagattccc
      961 ggctttgtgg agctcagcct gttcgaccaa gtgcggctct tggagagctg ttggatggag
     1021 gtgttaatga tggggctgat gtggcgctca attgaccacc ccggcaagct catctttgct
     1081 ccagatcttg ttctggacag ggatgagggg aaatgcgtag aaggaattct ggaaatcttt
     1141 gacatgctcc tggcaactac ttcaaggttt cgagagttaa aactccaaca caaagaatat
     1201 ctctgtgtca aggccatgat cctgctcaat tccagtatgt accctctggt cacagcgacc
     1261 caggatgctg acagcagccg gaagctggct cacttgctga acgccgtgac cgatgctttg
     1321 gtttgggtga ttgccaagag cggcatctcc tcccagcagc aatccatgcg cctggctaac
     1381 ctcctgatgc tcctgtccca cgtcaggcat gcgagatggg gagaaaagca attcattcat
     1441 ttgaagttat cttagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1501 tatatatctt gtggaaagga cgagtcgctg gagagcaggc tattgga
//
