LOCUS       sequence-279035-        2138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1963
FEATURES             Location/Qualifiers
     source          1..2138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggccaa tgtgggatcg atgtttcaat attggaagcg ctttgattta
       61 cagcagctgc agagagaact cgatgccacc gcaacggtat tggcgaaccg gcaggatgaa
      121 agtgagcagt ccagaaagcg gcttatcgaa cagagccggg agttcaagaa gaacactcca
      181 gaggatttgc gcaagcaggt agcgccgctg ctgaagagtt tccaaggaga gattgatgca
      241 ctgagtaaaa gaagcaagga agctgaagca gctttcttga atgtctacaa aagattgatt
      301 gacgtcccag atcccgtacc agctttggat ctcggacagc aactccagct caaagtgcag
      361 cgcctgcacg atattgaaac agagaaccag aaacttaggg aaactctgga agaatacaac
      421 aaggaatttg ctgaagtgaa aaatcaagag gttacgataa aagcacttaa agagaaaatc
      481 cgagaatatg aacagacact gaagaaccaa gccgaaacca tagctcttga gaaggaacag
      541 aagttacaga atgactttgc agaaaaggag agaaagctgc aggagacaca gatgtccacc
      601 acctcaaagc tggaggaagc tgagcataag gttcagagcc tacaaacagc cctggaaaaa
      661 actcgaacag aattatttga cctgaaaacc aaatacgatg aagaaactac tgcaaaggcc
      721 gacgagattg aaatgatcat gacggacctt gaaagggcaa accagagggc agaggtggct
      781 cagagagagg cggagacctt aagggaacag ctctcatcgg ccaatcactc cctccagctg
      841 gcctcacaga tccagaaggc accagacgtg gagcaggcca tagaggtgct gacccgctcc
      901 agcctagaag ttgagttggc cgccaaggag cgggagatcg cacagctggt ggaggacgtg
      961 cagagactcc aggccagcct caccaagctg cgggagaatt cggccagcca gatctcacag
     1021 cttgagcagc agctgagcgc caaaaacagc acactcaaac aactggaaga aaaactcaaa
     1081 ggccaggctg actatgaaga ggtgaagaaa gagctgaaca ttctgaagtc catggagttt
     1141 gcaccgtccg agggcgctgg gacacaggat gcggccaagc ccctggaggt gctgttgctg
     1201 gagaagaacc gctcgctgca gtccgagaac gccgcgctgc gcatctccaa cagcgacctg
     1261 agcggacgct gtgcggagct gcaagtccgt atcactgagg ctgtggccac agccactgag
     1321 cagagagagc tgatcgcccg cctggagcag gacctgagca tcattcagtc catccagcgg
     1381 cccgatgccg agggtgccgc tgagcaccgc ctggagaaga tcccagagcc catcaaagag
     1441 gccactgccc tattctacgg acctgcagca ccagccagcg gtgccctccc agagggccag
     1501 gtggattcac tgctttccat catctccagc cagagggagc gcttccgtgc ccggaaccag
     1561 gagcttgagg ccgagaaccg cctggcccag cacaccctcc aggccctgca gagtgagctg
     1621 gacagcctgc gcgccgacaa catcaagctc tttgagaaga tcaagttcct gcagagctac
     1681 cctggccggg gcagcggcag tgatgacacg gagctgcggt actcgtccca gtacgaggag
     1741 cgcctggacc ccttctcctc cttcagcaag cgggagcggc agaggaagta cctgagcttg
     1801 agtccctggg acaaggccac cctcagcatg gggcgtctgg ttctctccaa caagatggcg
     1861 cgcaccatcg gcttcttcta cacactgttc ctgcactgcc tggtcttcct ggtgctctac
     1921 aagctggcat ggagcgagag catggagagg gactgtgcca ccttctgcgc caagaagttc
     1981 gctgaccacc tgcacaagtt ccacgagaat gacaacgggg ctgcggctgg tgacttgtgg
     2041 cagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     2101 tgtggaaagg acgagtctgg tggagtctca tagagtta
//
