LOCUS       sequence-279071-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2018
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagttcgg cctgctcagc gaggcagagg cccggagccc tgccctgtcg
       61 ctgtcagacg ctggcactcc gcacccccag ctcccagagc acggctgcaa gggccaggag
      121 cacagcgact cagaaaaggc ctcggcttcg ctgcccggcg gctccccaga ggacggttcg
      181 ctgaaaaaga agcagcggcg gcagcgcacg cacttcacca gccagcagct acaggagcta
      241 gaggcgacct tccagaggaa ccgctacccc gacatgagca cgcgcgagga gatcgccgtg
      301 tggaccaacc tcaccgaggc ccgcgtgcgg gtgtggttca agaaccggcg cgccaaatgg
      361 cggaagcgcg agcgcagcca gcaggccgag ctatgcaaag gcagcttcgc ggcgccgctc
      421 ggggggctgg tgccgcccta cgaggaggtg taccccggct actcgtacgg caactggccg
      481 cccaaggctc ttgccccgcc gctcgccgcc aagacctttc cattcgcctt caactcggtc
      541 aacgtggggc ctctggcttc gcagcccgtc ttctcgccac ccagctccat cgccgcctcc
      601 atggtgccct ccgccgcggc tgccccgggc accgtgccag ggcctggggc cctgcagggc
      661 ctgggcgggg gcccccccgg gctggctccg gccgccgtgt cctccggggc cgtgtcctgc
      721 ccttatgcct cggccgccgc cgccgccgcg gctgccgcct cttcccccta cgtctatcgg
      781 gacccgtgta actcgagcct ggccagcctg cggctcaaag ccaaacagca cgcctccttc
      841 agctaccccg ctgtgcacgg gccgcccccg gcagccaacc ttagtccgtg ccagtacgcc
      901 gtggaaaggc ccgtatagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
      961 ctttatatat cttgtggaaa ggacgattgg aggcgggaat agcgaactgc
//
