LOCUS       sequence-279086-        1883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2043
FEATURES             Location/Qualifiers
     source          1..1883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1460..1469
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgaaactaca ggccgtgatg gagacgctgt tgcagcggca gcagcgggcg
       61 cgccaggagc tggaggcccg gcagcagctg ccccccgatc cccctgctgc accccccggc
      121 cgggcccggg ctgcccccga cgaggacaga gagcccgaga gtgcccggat gcagcgggct
      181 cagatggccg cactggcagc catgcgggct gcagctgcgg gcctgggaca cccagccagc
      241 cccggcggct ctgaggatgg gcccccaggc tcggaggagg aggacgcggc ccgggagggg
      301 acaccgggct cacccgggcg aggcagagaa gggccaggag aggagcactt tgaggacatg
      361 gcctccgacg aggacatgaa gcccaaatgg gaggaggagg agatggagga agacctcggg
      421 gaggatgagg aggaggagga ggaggattac gaggatgagg aggaggagga ggacgaggag
      481 gggctgggcc ccccaggccc tgccagcttg ggcaccacgg cactgttccc ccgaaaggcc
      541 cagccacccc aggccttccg cggcgatggc gttcccaggg tgctgggggg ccaggagcgg
      601 ccggggcctg gccctgccca ccccggaggg gccgcccacg tagccccgca gctgcagccg
      661 cctgaccacg gcgactggac ttacgaggag cagtttaagc agctctacga actcgacggg
      721 gaccccaaga ggaaggaatt cctggatgac ttgttcagct tcatgcagaa gcgagggaca
      781 cctgtgaacc gcatccccat catggccaaa caggtccttg acctgttcat gctgtacgtg
      841 ctggtgacgg agaagggcgg cctcgtggag gtcatcaaca agaagctgtg gcgtgagatc
      901 accaagggcc tcaacctgcc cacgtccatc accagtgcag ccttcaccct gcggacccaa
      961 tacatgaagt acctgtaccc ctacgagtgt gagaagcggg gcctcagtaa ccccaatgag
     1021 ctccaggcag ccatagacag caaccgacgg gagggccggc gccagagctt tggtggctcc
     1081 ctctttgcct actcgccagg cggggcacac ggcatgctct cctcacccaa gctacccgtg
     1141 tcctccctgg gcctggccgc aagcaccaat ggcagctcca tcacccccgc ccctaagatc
     1201 aagaaagagg aggactcagc catccccatc acagtccctg gccgcctgcc tgtgtccctg
     1261 gcgggccacc ctgtggtggc agcccaggca gcagctgtgc aagcagcagc cgcccaagca
     1321 gctgtggccg cacaggcagc tgccctggaa cagctgcggg agaagctgga gtctgcagag
     1381 cctccggaga agaagatggc cctggtggcc gatgagcagc aacggctgat gcaacgtgca
     1441 ctccagcaga acttcctggc catggcggcc cagctgccca tgagcattcg gatcaacagc
     1501 caagcctccg aaagccgcca ggactctgct gtgaacctga cgggcaccaa cggcagcaac
     1561 agcatcagca tgtcggtgga gatcaacggc atcatgtaca caggagttct gtttgctcag
     1621 ccgccggccc ccacgccaac ctctgctccc aacaaaggag gcggcggcgg cggcggcagc
     1681 agcagcaacg caggcggccg gggaggaaac accggaacca gcggcggcca ggctgggcca
     1741 gcggggctgt ccacaccctc cacatctacc tcaaataact cgttgcctta gtaatgaact
     1801 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgat
     1861 tagaggctgc cctctttagc cac
//
