LOCUS       sequence-279095-        1784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2521
FEATURES             Location/Qualifiers
     source          1..1784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggggcc gcagcccctg gcgctgcaac tggaacagtt gttgaacccg
       61 cgaccaagcg aggcggaccc tgaagcggac cccgaggaag ccactgctgc cagggtgatt
      121 gacaggtttg atgaagggga agatggggaa ggtgatttcc tagtagtggg tagcattaga
      181 aaactggcat cagcctccct cttggacacg gacaaaaggt attgcggcaa aaccacctct
      241 agaaaagcat ggaatgaaga ccattgggag cagactctgc caggatcgtc tgatgaggaa
      301 atatctgatg aggaagggtc tggagatgaa gattcagagg gactgggtct ggaggaatat
      361 gatgaggacg acctgggtgc tgctgaggaa caggagtgtg gtgatcacag ggagagcaag
      421 aagagcagaa gccactctgc aaaaacaccg ggcttcagtg tccagagtat cagtgacttt
      481 gagaaattta ccaagggaat ggatgacctt gggagcagtg aggaggagga agacgaagag
      541 agtggcatgg aagaagggga tgacgcggaa gactcccaag gcgagagtga ggaagacagg
      601 gctggagata gaaacagtga ggatgatggt gtggtgatga ccttctctag tgtcaaagtt
      661 tctgaggaag tggagaaagg aagagccgtg aagaaccaga tagcactgtg ggaccagctc
      721 ttggaaggaa ggatcaaact acaaaaagct ctgttgacca ccaaccagct tcctcaacca
      781 gatgttttcc cattgttcaa ggacaaaggt ggcccagaat tttccagtgc cctgaaaaat
      841 agtcacaagg cacttaaagc attgttgagg tcattggtag gtcttcagga agagttgctt
      901 ttccagtacc cagacactag atatctagta gatgggacaa agcccaatgc gggaagtgag
      961 gagatttcta gtgaagatga tgagctggta gaagagaaga agcagcaacg aagaagggtc
     1021 cctgcaaaga ggaagctgga gatggaggac tatcccagct tcatggcaaa gcgctttgcc
     1081 gactttacag tctacaggaa ccgcacactt cagaaatggc acgataagac caaactggct
     1141 tctggaaaac tggggaaggg ttttggtgcc tttgaacgct caatcttgac tcagatcgac
     1201 catattctga tggacaaaga gagattactt cgaaggacac agaccaagcg ctctgtctat
     1261 cgagttcttg gcaaacctga gccagcagct cagcctgtcc cagagagttt gccaggggaa
     1321 ccggagatcc ttcctcaagc ccctgctaat gctcatctga aggacttgga tgaagaaatc
     1381 tttgatgatg atgactttta ccaccagctc cttcgagaac tcatagaacg gaagaccagc
     1441 tccttggatc ccaacgatca ggtggccatg ggaaggcagt ggcttgcaat ccagaagtta
     1501 cgaagcaaaa tccacaaaaa agtagatagg aaagccagca aaggcaggaa acttcggttt
     1561 catgtcctta gcaagctact gagtttcatg gcacctattg accatactac aatgaatgat
     1621 gatgccagga cagaactgta ccgctctctt tttggccagc tccaccctcc cgacgaaggc
     1681 cacggggatt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     1741 atatcttgtg gaaaggacga aggagatgaa tgcctcctcc cgtg
//
