LOCUS       sequence-279134-        1256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2585
FEATURES             Location/Qualifiers
     source          1..1256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagggat cttagcctgg ttctggaacg agaggttttg gctcccgcac
       61 aatgtcacct gggcggacct gaagaacacg gaggaggcca ccttcccgca ggctgaggac
      121 ctctatctcg cttttcccct ggccttctgt atcttcatgg tgcggctcat cttcgagaga
      181 tttgtagcca aaccgtgcgc catagccctc aacattcagg ccaatggacc acaaattgct
      241 ccgcccaatg ccattctgga aaaggtcttc actgcaatta caaagcatcc tgatgaaaag
      301 agattggaag gcctctccaa gcaactggac tgggatgttc gaagcattca gcgctggttt
      361 cgacaaagac gcaatcagga gaagccaagc acgctgacga ggttctgtga gagcatgtgg
      421 agattttcat tttaccttta tgtatttacc tacggagtca gattcctgaa aaagaccccc
      481 tggttgtgga atacgaggca ttgctggtac aactacccct atcagccact cacaactgac
      541 cttcactact attacatcct ggagctgtcg ttttattggt ctttgatgtt ttctcagttc
      601 actgatatca aaagaaagga ctttggcatt atgttcctgc accaccttgt atctattttc
      661 ttgattacct tttcatatgt caacaatatg gcccgagtag gaacgctggt cctttgtctt
      721 catgattcag ctgatgctct tctggaggct gccaaaatgg caaattatgc caagtttcag
      781 aaaatgtgtg atctcctgtt tgttatgttt gccgtggttt ttatcaccac acgactgggt
      841 atatttcctc tctgggtgtt aaataccaca ttatttgaaa gctgggagat cgttggacct
      901 tacccttcct ggtgggtttt taacctactg ctattgctag tacaagggtt gaactgcttc
      961 tggtcttact tgattgtgaa aatagcttgc aaagctgttt caagaggcaa ggtgtccaag
     1021 gatgatcgaa gtgatattga gtctagctca gatgaggagg actcagaacc tccgggaaag
     1081 aatccccaca ctgcgacaac caccaatggg accagtggta ccaacgggta tctcctgact
     1141 ggctcctgct ccatggatga ttagtaatga actagtccgt aacttgaaag tatttcgatt
     1201 tcttggcttt atatatcttg tggaaaggac gacggagtct caggaggcct taggtt
//
