LOCUS       sequence-279143-        1940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2592
FEATURES             Location/Qualifiers
     source          1..1940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgctgg gcagcgggaa gcgaggcccc aggtgtcact gacattcgag
       61 gacgtggctg tgctctttac ctgggatgag tggagaaagc tggctccttc tcagagaaac
      121 ttgtaccggg atgtgatgct ggagaactat aggaacctgg tctcactggg actctcattt
      181 accaaaccaa aagtcatctc cctgttgcag caaggagaag atccctggga ggtggagaaa
      241 gacagttctg gtgtctcctc tctaggatgt aagagcacac ctaaaatgac aaagtcaact
      301 caaactcagg attcatttca ggagcagata aggaaaagat tgaaaaggga tgaaccctgg
      361 aacttcatat cagaaagatc ctgcatatat gaagagaaat taaagaaaca gcaggacaaa
      421 aatgaaaatt tacaaataat ttcagttgcc catacaaaaa tccttactgt agatagaagc
      481 cataaaaatg ttgaatttgg ccaaaacttc tacctgaaat cagtcttcat taagcaacag
      541 agatttgcta aagaaaaaac tccatcaaaa tgtgaaatac aaagaaatag tttcaagcag
      601 aattcaaatt tacttaacca atcaaaaatc aaaacagcag agaaacgcta taaatgcagt
      661 acatgtgaaa aagccttcat tcacaattca tcccttcgta aacatcagaa aaaccacact
      721 ggagaaaaat tatttaaatg taaagaatgt ttaaaagctt tcagccaaag ttctgctctt
      781 attcaacatc aaagaactca tacaggagag aaaccctata tatgtaaaga atgtgggaaa
      841 gccttcagcc atagtgcatc cctttgtaag catttaagga cccatactgt ggagaaatgc
      901 tatagatgta aagaatgtgg taaatccttc agtcgaaggt ctgggctttt tatacatcaa
      961 aaaatccatg ctcaagaaaa tccccataaa tacaatccag gcaggaaggc atccagttac
     1021 agcacttccc tttctggaag tcagaaaatt catctcagaa agaagtccta cttatgtaat
     1081 gaatgtggca acacctttaa gtctagctca tcccttcgtt atcatcagag aattcacact
     1141 ggagagaagc cttttaaatg tagtgaatgt gggagagcct tcagccagag tgcctctctt
     1201 attcaacatg aaagaattca caccggagaa aagccctata gatgcaatga atgtgggaaa
     1261 ggctttactt ctatttcacg acttaataga caccgaataa ttcatactgg agagaaattg
     1321 tataattgta atgaatgtgg taaagcctta agctcccact caacacttat tattcatgag
     1381 cgaattcata ctggagaaaa accatgtaaa tgtaaagtat gtggaaaagc cttcagacag
     1441 agttccgctc tcattcaaca tcagagaatg catactggag aaagacccta taagtgtaac
     1501 gaatgtgaca aaacattcag gtgtaactca tcgcttagta atcaccagag aattcatact
     1561 ggagagaaac catatcgatg tttagaatgt gggatgtctt ttggccaaag tgcagctctt
     1621 atacaacatc agaggattca tacaggagaa aaacccttta aatgtaatac atgtggaaaa
     1681 acttttagac aaagctcatc acttattgca catcaaagaa ttcatactgg agagaaaccc
     1741 tatgaatgta atgcatgtgg gaaactcttt agccagaggt catcccttac taatcattat
     1801 aaaattcaca ttgaagagga ctccttaaaa gccgatttgc atgtgtagta atgaactagt
     1861 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaatgt
     1921 ctgcgaagta ctccctattt
//
