LOCUS       sequence-279146-        1340 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2593
FEATURES             Location/Qualifiers
     source          1..1340
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcggat ccccagcgcg caaccacccc ccgcctgcct gcccaaagca
       61 ccgggactgg agcacggaga cctaccaggg atgtaccctg cccacatgta ccaagtgtac
      121 aagtcaagac ggggaataaa gcggagcgag gacagcaagg agacctacaa attgccgcac
      181 cggctcatcg agaaaaagag acgtgaccgg attaacgagt gcatcgccca gctgaaggat
      241 ctcctacccg aacatctcaa acttacaact ttgggtcact tggaaaaagc agtggttctt
      301 gaacttacct tgaagcatgt gaaagcacta acaaacctaa ttgatcagca gcagcagaaa
      361 atcattgccc tgcagagtgg tttacaagct ggtgagctgt cagggagaaa tgtcgaaaca
      421 ggtcaagaga tgttctgctc aggtttccag acatgtgccc gggaggtgct tcagtatctg
      481 gccaagcacg agaacactcg ggacctgaag tcttcgcagc ttgtcaccca cctccaccgg
      541 gtggtctcgg agctgctgca gggtggtacc tccaggaagc catcagaccc agctcccaaa
      601 gtgatggact tcaaggaaaa acccagctct ccggccaaag gttcggaagg tcctgggaaa
      661 aactgcgtgc cagtcatcca gcggactttc gctcactcga gtggggagca gagcggcagc
      721 gacacggaca cagacagtgg ctatggagga gaatcggaga agggcgactt gcgcagtgag
      781 cagccgtgct tcaaaagtga ccacggacgc aggttcacga tgggagaaag gatcggcgca
      841 attaagcaag agtccgaaga accccccaca aaaaagaacc ggatgcagct ttcggatgat
      901 gaaggccatt tcactagcag tgacctgatc agctccccgt tcctgggccc acacccacac
      961 cagcctcctt tctgcctgcc cttctacctg atcccacctt cagcgactgc ctacctgccc
     1021 atgctggaga agtgctggta tcccacctca gtgccagtgc tatacccagg cctcaacgcc
     1081 tctgccgcag ccctctctag cttcatgaac ccagacaaga tctcggctcc cttgctcatg
     1141 ccccagagac tcccttctcc cttgccagct catccgtccg tcgactcttc tgtcttgctc
     1201 caagctctga agccaatccc ccctttaaac ttagaaacca aagactagta atgaactagt
     1261 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacagt
     1321 aatcctacgt aatgtgggcc
//
