LOCUS       sequence-279182-        1310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2649
FEATURES             Location/Qualifiers
     source          1..1310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcagc agccgctctg agggcgcctg ctcagagcag tgtgaccttt
       61 gaagatgtgg ctgtaaactt ttccctggag gaatggagtc ttcttaatga ggctcagaga
      121 tgcctgtacc gtgacgtgat gctggagacc ttgacactta tatcctccct gggttgttgg
      181 catggagggg aagatgaggc agcaccttct aagcagagca cgtgtataca tatatacaaa
      241 gaccagggag gtcatagtgg agaaaggcct tatgagtgtg gggaatatag gaaattattt
      301 aagaacaagt cctgcctcac tgaacccaga agagatcaca aacacaggaa tgttcgcact
      361 ggagaaaggc cttatgagtg cagcaaatat gggaaattat ttcaccaaaa gcctacactc
      421 catattcatg agagatttca tactgggcaa aagacctatg agtgcagtga gtgtggaaaa
      481 tcatttcacc aaagctcttc actcttgcag cgtcagacac ttcacactag agaaaggcct
      541 tatgagtgta ttgaatgtgg gaaagccttt gctgaaaagt ccagtctcat taaccacagg
      601 aaagttcact ctggagcaaa gcgttatgaa tgcaatgaat gtgggaagtc ctttgcttat
      661 acatctagtc tcattaaaca caggaggatt cacactggag agaggcctta tgagtgcagt
      721 gaatgtggga gatcctttgc tgaaaactcc agtcttatta aacacttgag agttcacaca
      781 ggagaaaggc cttatgaatg cgttgagtgt ggaaaatcat ttcgccgaag ctcttcactc
      841 ttgcagcatc agagagttca cactagagaa aggccttatg aatgtagtga atgtgggaaa
      901 tcctttagct taaggtccaa cctcattcac catcagcgag ttcatactgg agaaaggcat
      961 gagtgcgggc agtgtgggaa atcctttagc cgaaaatcta gccttattat acatctgaga
     1021 gttcacactg gagaaaggcc ttatgagtgc agtgactgtg ggaaatcctt tgctgaaaac
     1081 tccagcctta ttaaacactt gagagttcac actggagaaa ggccatatga atgcattgat
     1141 tgtggaaaat catttcgcca cagttcttcg ttccgtcgcc atcagagagt tcatactgga
     1201 atgaggcctt ataagtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1261 ctttatatat cttgtggaaa ggacgaggag cgctagaatg gtccgtctca
//
