LOCUS       sequence-279221-         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0186
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgcccc tcccaaagct cccatccgtg tcaggaattt gaccatcaga
       61 gcaggagccc tcactgggaa ggagaacaac atgctgcagc ccgagaccca catcttcaca
      121 gcccccgagg aggggagctc ccaaggggct ctgctgcttg gccaggcccc agaacctttg
      181 tctctgccct gtactccagt gaccttggag cagcaactgg tttctccttc tggggatccc
      241 cacagggccc ctgccctgcc cagcatatgc tcaactctga tcctccagcc ctgtgccacc
      301 cttgaccctc ttctactgca gccacaggtc cccaaagtct ctgaccaggc tttggtttct
      361 gcccactcag agtggcagcg gaagctggag gccgctgagg ctctgctgac tctgagaaac
      421 tctgcccagg cccctcctga ctccatctcc ctgcaccagc cttgcaaccc accagctcct
      481 gctggagata aaggattcca gcctcccagc ccctctctcc gccccaggcc agccagctcc
      541 atctcgctgc ctattggaca tctgggatgc atctccctct tgagctagta atgaactagt
      601 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaggcg
      661 tcgacgaatt tagttgagaa
//
