LOCUS       sequence-279224-        1358 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0187
FEATURES             Location/Qualifiers
     source          1..1358
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaacccag tgacatgcct gctggctacc actgcccctt agactctgcc
       61 ccctgggatg agaccagaga cccccagagc acagagctga tccccaggag agccatcagc
      121 cgctctccaa cctgcgcccg ctgccgcaac catggtgtca ccgcccatct caagggccac
      181 aagcgcctct gcctcttcca ggcttgcgag tgtcacaaat gtgtcctcat cctggagcgc
      241 cgcagggtca tggctgccca ggtggccttg cgtaggcagc aggaggcgca gctaaagaag
      301 cacctgatga ggagagggga agcctctccc aaagctccca accacttcag aaagggaacc
      361 actcagccac aggtcccctc tggaaaggag aacatagcac cccagcctca gaccccccat
      421 ggggcagtcc tgctggcacc gacacccccc gggaagaact cctgtgggcc tctgctgctc
      481 agccatcccc cggaagcctc gcccttgtcc tggactccgg tgcctcctgg cccttgggtc
      541 cctggacact ggctgcctcc aggcttctcc atgccaccac cagtggtgtg ccgcctgctg
      601 taccaagaac ctgctgtctc tctgcctccc ttccctggct ttgaccctgg cacctccctc
      661 cagctgccca ctcatgggcc cttcaccacc tgcccaggat ctcacccagt actgacagct
      721 cctctttctg gagagcccca agggccccct agccagcccc gcacacactc aactctgata
      781 ctccagccct gtggcacccc agaccctctt cagctacagc cacaggtcct gggaaagaag
      841 tgggatctag ggccctggga ggaggttgag cctggggaag gaaaaagcag gaaactgaag
      901 gaagcagggg ctgaggaaca cttagagggt gggcagaagc gggggttccc agtctgcttg
      961 tctccctttc ccttgcaggc ctctggagcc tcgtgcctgg cccggacatc tggcccctca
     1021 gagtggcagc tgcagcaaga ggcagctgaa gccctcgtgg ggctgaaaga ttcatcccag
     1081 gctcctcgtg tgaccccttc tgtgcccccc aaccctgcct ggatctccct gcttcacccc
     1141 tgtggcccac cagctcctgc tggaggaaga ggattccagc ctgttggccc ctgtcttcga
     1201 cccagcccag ccccctctgt tgctctgcat attggccgtc tggggtccat ctccctcctg
     1261 agctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1321 tgtggaaagg acgaaccgta gtcagccaac cattgcaa
//
