LOCUS       sequence-279227-        2888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0194
FEATURES             Location/Qualifiers
     source          1..2888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccaaggt cccggagctg gaagacacct tcctgcaggc gcagcctgcg
       61 ccccaactgt ccccggggat ccaggaagac tgctgtgtgc agctcctggg caagggcttg
      121 ctagtctatc cggaagaaac agtgtacctg gcggccgaag ggcagcccgg gggcgagcag
      181 ggcggcgggg agaaaggcga agacccggag ctgccggggg cagtgaaatc agaaatgcac
      241 ttaaacaatg gtaacttttc ctctgaagaa gaggacgccg acaaccacga cagcaaaacc
      301 aaagcagcgg atcaatacct gtctcagaag aaaaccatca cgcagattgt gaaggataaa
      361 aagaagcaga cacagctcac gctgcagtgg cttgaagaga attacattgt atgtgaagga
      421 gtttgcttac cacggtgcat tctttatgca cactacttag atttctgtag gaaagagaaa
      481 ttagagccag cctgtgcggc cacctttgga aagacaattc gccagaagtt tcccctccta
      541 acaacaaggc ggcttggaac aagaggccat tcaaagtatc attactatgg gattggcatc
      601 aaagagagca gtgcatatta ccactccgtt tattctggaa agggcttgac aaggttttct
      661 ggaagcaagc taaagaatga gggtggcttc actcgtaaat attcgcttag ctcaaaaact
      721 ggaacacttc ttccagaatt ccccagcgct caacaccttg tataccaagg atgcatttct
      781 aaggacaagg ttgatacgct cataatgatg tacaaaactc actgccagtg tatcctggac
      841 aatgcaatta atggaaactt tgaagagatc cagcattttt tattacactt ttggcaagga
      901 atgcctgacc atctccttcc cctgctcgaa aatcctgtta tcattgatat tttctgtgtt
      961 tgtgactcaa ttctttataa ggttcttaca gatgtactca ttcctgcaac aatgcaagaa
     1021 atgcctgaaa gcttattagc agacataaga aattttgcta aaaattggga acagtgggtt
     1081 gtttcatcct tggaaaactt gccagaagct ctaactgaca agaaaatacc tattgtgcga
     1141 agatttgtat cttctctgaa acgacaaaca tctttcttac atcttgccca gattgccaga
     1201 ccagctctct ttgaccagca tgtcgttaat tctatggtgt ctgatattga aagggttgat
     1261 ttgaacagca ttggctctca agcccttctt accatttcag gcagcacaga cactgaatct
     1321 ggtatctaca ctgaacatga ctctatcact gtgttccaag aactgaagga tctccttaag
     1381 aagaatgcca ctgtggaggc ttttattgaa tggttggata ctgtggtaga acagagagtt
     1441 attaagacca gcaaacaaaa tggaaggtca ttaaagaaga gagctcaaga ctttctgtta
     1501 aagtggagtt tttttggtgc tcgagtaatg cataatctca ccttgaacaa tgcatccagt
     1561 tttggttctt ttcatttgat tcgaatgctt ctcgatgaat acattctcct ggccatggag
     1621 acccagttta ataatgacaa agagcaggag ttacagaatt tattggacaa gtatatgaag
     1681 aattcagatg cgagtaaagc tgctttcact gcttctccga gttcatgctt tctggccaac
     1741 cgtaataaag ggagcatggt ttccagcgac gctgtgaaga atgaaagcca cgtggagaca
     1801 acctatctcc ctctgccatc cagtcaacct ggaggcctag gccctgctct gcaccagttc
     1861 cctgctggga acacagacaa catgccgctc acaggtcaaa tggagctttc acagattgct
     1921 ggtcatctga tgacaccacc catttctcca gccatggcaa gccgaggaag tgtcattaac
     1981 caaggaccaa tggcagggag gcccccaagt gtgggcccag tactgtcagc tccatcacac
     2041 tgctccacat acccagagcc catttatccc actctccctc aagccaatca tgacttttat
     2101 agcaccagct ctaactacca gactgtgttt agggcacagc cccactccac atcaggactc
     2161 tatcctcatc acaccgagca tggtcgatgc atggcttgga ctgaacagca gctttcaaga
     2221 gacttcttca gtggcagctg tgcggggtct ccatataact cccggccacc gtctagctat
     2281 ggcccatccc tgcaagccca ggattcacac aatatgcagt ttttaaatac aggaagcttc
     2341 aatttcttga gcaacacagg agctgccagc tgccaaggag caacactgcc tcctaattca
     2401 ccaaatggat actatggaag caacataaac tacccagagt ctcacaggct cggatcaatg
     2461 gtgaatcagc acgtttctgt catcagcagc attcgttcac tgccccccta cagtgacatc
     2521 cacgatccac ttaacatttt agatgacagt ggtagaaaac agaccagctc gttttacaca
     2581 gacacatcat ctccagttgc atgtcgaact ccagtcctag cttccagttt gcaaacccca
     2641 attccttctt cctcatccca atgtatgtat ggaacttcca accagtatcc agctcaagaa
     2701 accctggact cccatggaac aagcagtaga gaaatggtgt cctctttacc acctatcaac
     2761 actgtgttca tgggaacagc agctggaggc acttagtaat gaactagtcc gtaacttgaa
     2821 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaattggt ctcttacatg
     2881 tggtctgt
//
