LOCUS       sequence-27923-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Todi SV, Laco MN, Winborn BJ, Travis SM, Wen HM, Paulson HL
  TITLE     Cellular turnover of the polyglutamine disease protein ataxin-3 is 
            regulated by its catalytic activity.
  JOURNAL   J Biol Chem. 2007 Oct 5. 282(40):29348-58.
  PUBMED    17693639
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Oct 5. 282(40):29348-58."
COMMENT     Sequence Label: pVETL-Cmcs-Flag-Ataxin3Q25
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(536..559)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(662..700)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gcttcgcatc tggcagatca cccttaacaa caaatataga ataaccttcc tgttgtaatt
       61 gagccaagaa aagtgcaaga tatgtatctg atattaattc tggacccgtc aagagagaat
      121 tcaagttaaa ccactgtttt cctaattttc taactgtaaa ccagtgttcc ttataattgc
      181 atataaatga tctttcattt ataggatcga tcctgagcct ctgatactct ggactgttga
      241 acaggattag ttctaaaccc caaactttca aggcattgct tataacctga atagagaaaa
      301 aaccactgtc atccatattt ccagaaggct gctgtaaaaa cgtgcgataa tcttcactag
      361 taactcctcc ttctgccatt ctcatcctct cctcctcatc cagctgatgt gcaattgagg
      421 ataattccac agggctaaaa tattctcctt gcaataagtt attcaggcaa tgttgagcac
      481 aaagtgagcc ttcttgtttc tcgtggaaga tggactctcc gtttataagc ttgagcttgt
      541 cgtcatcgtc tttgtagtcc atagctctag agtccggagt gcgcgnccgn ccggggtntc
      601 ttnngnnggg agtcaaaaca gcgtggatgg cgtctccggg cgatctgacg ntcnataaac
      661 gagctctgct tatatagacc tcccacngta cacgcctacc gnccntt
//
