LOCUS       sequence-279230-        3905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0224
FEATURES             Location/Qualifiers
     source          1..3905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggggatat gaaaacccca gattttgatg accttctggc tgcctttgac
       61 atcccagacc ccaccagcct tgatgccaag gaggccatcc agacacccag tgaggagaat
      121 gagagtcccc tcaaacctcc aggcatatgt atggatgaaa gtgtgtcctt gtctcactca
      181 ggatcagccc ccgatgtgcc ggccgtgagt gtcattgtca agaacaccag ccgccaggag
      241 tcatttgaag cggagaaaga ccacattact cccagtctcc tacacaatgg attccggggc
      301 tcagatctgc ctccagatcc ccacaactgt gggaaatttg attctacttt tatgaatgga
      361 gacagtgcca ggagtttccc tggcaaactg gagcctccca agtcagagcc attacccacc
      421 ttcaaccagt tcagtccaat ctccagccca gaacctgagg atcccatcaa agataacgga
      481 tttgggataa agcccaaaca ctctgacagt tatttcccac cccctcttgg gtgcggggct
      541 gtgggaggcc cagtcctgga ggctctggct aagtttccgg ttccagagct gcatatgttt
      601 gatcattttt gtaagaaaga acccaagcca gaacccctgc ccttggggag ccagcaggaa
      661 cacgagcaaa gtgggcagaa cacagtggaa cctcacaagg atccggatgc cactcgattc
      721 ttcggggaag ctttggagtt caacagccat cctagcaaca gtattggaga gtccaagggg
      781 cttgcccggg agcttggtac ctgctcatca gtccccccta ggcagcgtct aaagccagct
      841 cattccaagc tgtcctcttg tgtggcagcc ttggtggcct tgcaggccaa aagagtggct
      901 agtgtcacta aggaggatca gcctggccac acaaaggatc tctcagggcc cactaaagag
      961 agttctaaag gtagccccaa aatgcccaag tcaccaaaga gtccccggag ccctctggag
     1021 gccactagaa aaagtatcaa gccatcggac agccctcgta gcatctgcag tgacagcagc
     1081 agcaaaggct caccgtctgt ggctgccagc tccccaccag caattcccaa agtgagaatc
     1141 aaaaccatta agacatcatc aggggaaatc aaacggactg tcacaaggat cctgccagat
     1201 cctgatgatc caagtaagtc ccctgttggg tcacctctag ggagcgccat tgcagaggcc
     1261 cccagcgaga tgccagggga tgaggtgcct gtggaagagc actttcctga ggcaggcaca
     1321 aattcaggga gcccccaggg ggccaggaaa ggggacgaga gcatgacaaa ggccagtgac
     1381 tcgtcatctc ccagctgcag ttctgggccc cgggtcccaa agggggctgc cccaggctca
     1441 cagacaggca agaagcaaca gagcacagca ctgcaggcat ccaccctggc ccctgccaac
     1501 ctcctgccca aagccgtgca cttggccaac ctgaacctcg tcccccacag tgttgctgca
     1561 tcagtgacag ccaagtcttc agtgcaaaga cggagccagc cacagcttac acaaatgtcg
     1621 gtgcccctgg tccaccaggt gaaaaaggct gccccactga ttgtagaggt cttcaacaag
     1681 gtccttcaca gctccaaccc cgtgcccctc tatgcgccaa atctcagccc gcctgcggac
     1741 agcaggatcc acgtgccggc cagtgggtac tgctgcctgg agtgtggaga cgcatttgcc
     1801 ttagagaaga gcctgagcca gcactatggc cggcggagcg tccacattga ggtactgtgc
     1861 acactgtgct ccaagacgct gctcttcttc aacaagtgca gcctgctccg gcacgcccgt
     1921 gaccacaaga gcaaggggct cgtcatgcag tgttcccagc tgctggtgaa gcctatctct
     1981 gcggaccaaa tgttcgtgtc ggcccctgtg aactccacgg caccagcagc cccagcccct
     2041 tcatcctctc ccaaacatgg cctcacttcg ggcagtgcca gtccccctcc tccagccttg
     2101 ccactctacc cagaccctgt gaggctcatc cggtactcaa tcaagtgtct tgaatgtcac
     2161 aagcagatgc gggactacat ggtcctggct gcacatttcc agaggacaac agaggagaca
     2221 gaggggctga cctgccaggt atgccaaatg ctgctgccca accagtgcag tttctgtgcc
     2281 caccagcgga ttcatgcaca caagtccccc tactgctgcc cggagtgtgg ggtcctctgc
     2341 cgctctgcct acttccagac ccatgtaaag gagaattgcc tgcactatgc ccgcaaggtg
     2401 ggctacaggt gcatccactg tggtgtcgtc cacctgacct tggccttgct gaaaagccac
     2461 atccaggagc gacactgcca ggttttccac aaatgtgcat tctgccccat ggccttcaag
     2521 actgccagca gcactgcaga ccacagtgcc acccagcacc ccacccagcc ccacagaccc
     2581 tcccagctca tttataagtg ctcctgtgaa atggtcttca acaagaagag gcacattcag
     2641 cagcattttt accagaatgt cagcaagacg caggtgggcg tcttcaagtg ccctgagtgc
     2701 ccactcttgt tcgtgcagaa gccggagttg atgcaacacg tcaagagcac ccacggtgtt
     2761 ccccgaaatg tggacgagct gtcaagcctc cagtcttcag cggacacatc ctcaagccgc
     2821 cctggctctc gagttcccac tgagccacca gccactagtg tggctgctcg gagcagctcc
     2881 ctgccttctg gccgctgggg taggcctgaa gcccaccgca gggtggaagc caggccgcgg
     2941 ctgaggaaca ctggctggac ctgccaggag tgccaggagt gggttccaga tcgggagagc
     3001 tacgtgtccc acatgaaaaa gagccacggt cggacattga agcggtaccc atgccggcag
     3061 tgtgaacagt ccttccacac ccccaacagc ctgcgcaaac acatccgcaa caaccatgac
     3121 acagtaaaga agttctacac ctgcgggtac tgcacagagg acagccccag ctttcctcgg
     3181 ccctcccttc tggagagcca catcagcctt atgcatggca tcagaaaccc tgatttgagc
     3241 cagacgtcca aagtgaaacc tccgggtgga cattcccctc aggtgaacca tctgaaaaga
     3301 ccagtcagtg gagtggggga cgctccaggc accagcaatg gcgcaactgt ctcttccacc
     3361 aaaaggcaca agtccctttt tcagtgcgcg aaatgtagtt ttgccacaga ctcggggctc
     3421 gagtttcaga gccacatacc tcagcaccag gtggacagct ccacagccca atgtctcctc
     3481 tgtggtttgt gctacacctc tgccagctcc ctcagccgcc acctcttcat tgtccacaag
     3541 gtgagagacc aggaggagga ggaggaagag gaggcggcgg cagcggagat ggcagtggag
     3601 gtggcagagc cagaggaggg ctccggggag gaggtgccca tggagactag agagaatgga
     3661 ctggaagaat gtgccggtga gcctttgtca gctgacccag aggcgaggag attgctgggc
     3721 ccggcccctg aggacgatgg tggccacaat gatcacagtc aaccacaggc ctctcaggac
     3781 caggacagcc acacactgtc ccctcaggtg tagtaatgaa ctagtccgta acttgaaagt
     3841 atttcgattt cttggcttta tatatcttgt ggaaaggacg acccgggtcc agtgagagct
     3901 tgcag
//
