LOCUS       sequence-279233-         572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0225
FEATURES             Location/Qualifiers
     source          1..572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtcgctc ccgccggaca ggcgcgcacc gagcgcactc tctagcccgg
       61 cagatgaagg cgaagcggcg gcggccggac ttggatgaga ttcaccgcga gctgcggcct
      121 cagggatccg cacgacccca gcccgaccca aacgccgagt tcgaccccga cctgccaggg
      181 ggcggtctgc accgctgtct ggcctgcgcg aggtacttca tcgattccac caacctgaag
      241 acccacttcc gatccaaaga ccacaagaaa aggtatgaag gagtaaggag aggattgatg
      301 gatgggtgct cagcagatag ggctctcacc tggtcagctc ccaactcctg tttttctttc
      361 tcccaggctg aagcagctga gcgtcgagcc ctacagtcag gaagaggcgg agagggcagc
      421 gggtatggga tcctatgtgc cccccaggcg gctggcagtg cccacggaag tgtccactag
      481 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      541 aaggacgagc tagctcggtt caggcctcga ag
//
