LOCUS       sequence-279242-        3668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1662
FEATURES             Location/Qualifiers
     source          1..3668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcccaa ctcactccag tgggtcggct caccgtgtgg cttgcacgga
       61 ccttacattt tctacaaggc ttttcaattc caccttgaag gcaaaccaag aattttgtcc
      121 cttggcgact ttttctttgt aagatgtacg ccaaaggatc cgatttgcat agcggagctc
      181 cagctgttgt gggaagagag gaccagccgg caacttttat ccagctctaa actttatttc
      241 ctcccagaag acactcccca gggcagaaat agcgaccatg gcgaggatga agtcattgct
      301 gtttccgaaa aggtgattgt gaagcttgaa gacctggtca agtgggtaca ttctgatttc
      361 tccaagtgga gatgtggctt ccacgctgga ccagtgaaaa ctgaggcctt gggaaggaat
      421 ggacagaagg aagctctgct gaagtacagg cagtcaaccc taaacagtgg actcaacttc
      481 aaagacgttc tcaaggagaa ggcagacctg ggggaggacg aggaagaaac gaacgtgata
      541 gttctcagct acccccagta ctgccggtac cgctcgatgc tgaaacgcat ccaggataag
      601 ccatcttcca ttctaacgga ccagtttgca ttggccctgg ggggcattgc agtggtcagc
      661 aggaaccctc agatcctgta ctgtcgggac acctttgacc acccgactct catagaaaac
      721 gagagtatat gcgatgagtt tgcgccaaat cttaaaggca gaccacgcaa aaagaaacca
      781 tgcccacaaa gaagagattc attcagtggt gttaaggatt ccaacaacaa ttccgatggc
      841 aaagccgttg ccaaggtgaa atgtgaggcc aggtcagcct tgaccaagcc gaagaataac
      901 cataactgta aaaaagtctc aaatgaagaa aaaccaaagg ttgccattgg tgaagagtgc
      961 agggcagatg aacaagcctt cttggtggca ctttataaat acatgaaaga aaggaaaacg
     1021 ccgatagaac gaatacccta tttaggtttt aaacagatta acctttggac tatgtttcaa
     1081 gctgctcaaa aactgggagg atatgaaaca ataacagccc gccgtcagtg gaaacatatt
     1141 tatgatgaat taggcggtaa tcctgggagc accagcgctg ccacttgtac ccgcagacat
     1201 tatgaaagat taatcctacc atatgaaaga tttattaaag gagaagaaga taagcccctg
     1261 cctccaatca aacctcggaa acaggagaac agttcacagg aaaatgagaa caaaacaaaa
     1321 gtatctggaa ccaaacgcat caaacatgaa atacctaaaa gcaagaaaga aaaagaaaat
     1381 gccccaaagc cccaggatgc agcagaggtt tcatcagagc aagaaaaaga acaagagact
     1441 ttaataagcc agaaaagcat ccctgagcct ctcccagcag cagacatgaa gaaaaaaata
     1501 gaagggtatc aggaattttc agcgaagccc ctggcatcca gagtagaccc agagaaggac
     1561 aacgaaacag accaaggttc caacagtgag aaggtggcag aggaggcggg agagaagggg
     1621 cccacacctc cactcccaag tgctcctctg gccccagaaa aagattcagc cttggtccct
     1681 ggggccagca aacagccact cacctctcct agtgccctgg tggactcaaa acaagaatcc
     1741 aaactgtgct gttttacaga gagccctgaa agtgaacccc aagaagcatc cttccccagc
     1801 ttccccacca cacagccacc gctggcaaac cagaatgaga cggaggatga caaactgccc
     1861 gccatggcag attacattgc caactgcacc gtgaaggtgg accagctggg cagtgacgac
     1921 atccacaatg cgctcaagca gaccccaaag gtccttgtgg tccagtcgtt tgacatgttc
     1981 aaagacaaag acctgactgg gcccatgaac gagaaccatg gacttaatta cacgcccctg
     2041 ctctactcta ggggcaaccc aggcatcatg tccccactgg ccaagaaaaa gcttttgtcc
     2101 caagtgagtg gggccagcct ctccagcagc tacccttatg gctccccacc ccctttgatc
     2161 agcaaaaaga aactgattgc tagggatgac ttgtgttcca gtttgtccca gacccaccat
     2221 ggccaaagca ctgaccatat ggcggtcagc cggccatcag tgattcagca cgtccagagt
     2281 ttcagaagca agccctcgga agagagaaag accatcaatg acatctttaa gcatgagaaa
     2341 ctgagtcgat cagatcccca ccgctgcagc ttctccaagc atcaccttaa cccccttgct
     2401 gactcctacg tcctgaagca agaaattcag gagggcaagg ataaactctt agagaaaagg
     2461 gccctccccc attcccacat gcctagcttc ctggctgact tctactcgtc ccctcatctc
     2521 catagcctct acagacacac cgagcaccat cttcataatg aacagacatc caaataccct
     2581 tccagggaca tgtacaggga atcggaaaac agttcttttc cttcccacag acaccaagaa
     2641 aagctccatg taaattatct cacgtccctg cacctgcaag acaaaaagtc ggcggcagca
     2701 gaagccccta cggatgatca gcctacagat ctgagccttc ccaagaaccc gcacaaacct
     2761 accggcaagg tcctgggcct ggctcattcc accacagggc cccaggagag caaaggcatc
     2821 tcccagttcc aggtcttagg cagccagagt cgagactgtc accccaaagc ctgtcgggta
     2881 tcacccatga ccatgtcagg ccctaaaaaa taccctgaat cgctttcaag atcaggaaaa
     2941 cctcaccatg tgagactgga gaatttcagg aagatggaag gcatggtcca cccaatcctg
     3001 caccggaaaa tgagcccgca gaacattggg gcggcgcggc cgatcaagcg cagcctggag
     3061 gatttggacc ttgtgattgc agggaaaaag gcccgggcag tgtctccctt agacccatcc
     3121 aaggaggtct ctgggaagga gaaggcctct gagcaggaga gtgaaggcag caaagcagcg
     3181 cacggtgggc attccggggg cggatcagaa ggccacaagc ttcccctctc ctcccctatc
     3241 ttcccaggtc tgtattccgg gagcctgtgt aactcgggcc tcaactccag gctcccggct
     3301 gggtattctc attctctgca gtacttgaaa aaccagactg tgctttctcc actcatgcag
     3361 cccctggctt tccactcgct tgtgatgcaa agaggaattt ttacatcacc gacaaattct
     3421 cagcagctgt acagacactt ggctgcggct acacctgtag gaagttcata tggggacctt
     3481 ttgcataaca gcatttaccc tttagctgct ataaatcctc aagctgcctt tccatcttcc
     3541 cagctgtcat ccgtgcaccc cagtacaaaa ctgtagtaat gaactagtcc gtaacttgaa
     3601 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatcagct acttccttta
     3661 caggtccc
//
