LOCUS       sequence-27925-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Todi SV, Laco MN, Winborn BJ, Travis SM, Wen HM, Paulson HL
  TITLE     Cellular turnover of the polyglutamine disease protein ataxin-3 is 
            regulated by its catalytic activity.
  JOURNAL   J Biol Chem. 2007 Oct 5. 282(40):29348-58.
  PUBMED    17693639
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Oct 5. 282(40):29348-58."
COMMENT     Sequence Label: pVETL-Cmcs-Flag-Ataxin3Q22-C14A
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(537..560)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     gap             644..655
                     /estimated_length=12
     primer_bind     complement(691..711)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gcttcgcatc tggcagatca cccttaacaa caaatataga ataaccttcc tgttgtaatt
       61 gagccaagaa aagtgcaaga tatgtatctg atattaattc tggacccgtc aagagagaat
      121 tcaagttaaa ccactgnttt tcctaatttt ctaactgtaa accagtgttc cttataattg
      181 catataaatg atctttcatt tataggatcg atcctgagcc tctgatactc tggactgttg
      241 aacaggatta gttctaaacc ccaaactttc aaggcattgc ttataacctg aatagagaaa
      301 aaaccactgt catccatatt tccagaaggc tgctgtaaaa acgtgcgata atcttcacta
      361 gtaactcctc cttctgccat tctcatcctc tcctcctcat ccagctgatg tgcaattgag
      421 gataattcca cagggctaaa atattctcct tgcaataagt tattcaggca atgttgagca
      481 gcaagtgagc cttcttgttt ctcgtggaag atggactctc cgtttataag cttgagcttg
      541 tcgtcatcgt ctttgtagtc catagctcta gagtccggag gctggatcgg tcccggtgtc
      601 ttctatggag gtcaaaacag cgtggatggc gtctccgang nagnnnnnnn nnnnnggaag
      661 actngctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
      721 cggagttgtt acgacatttt ggaagtccng tgatttggtg ccaacaactc catgcgtcca
      781 tgggtgggac tggannccgn agcaacgctt cccgccatga gtctgcaacc caccctgga
//
