LOCUS       sequence-279251-        3842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1307
FEATURES             Location/Qualifiers
     source          1..3842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1266..1274
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgagcaagga aagacccaag aggaatatca ttcagaagaa atacgatgac
       61 agtgatggga ttccgtggtc agaagaacgg gtggtacgta aagtccttta tttgtctctg
      121 aaggagttca agaattccca gaagaggcag catgcggaag gcattgctgg gagcctgaaa
      181 actgtgaatg ggctccttgg taatgaccag tctaagggat taggaccagc atcagaacag
      241 tcagagaatg aaaaggacga tgcatcccaa gtgtcctcca ctagcaacga tgttagttct
      301 tcagattttg aagaagggcc gtcgaggaaa aggcccaggc tgcaagcaca aaggaagttt
      361 gctcagtctc agccgaatag tcccagcaca actccagtaa agatagtgga gccattgcta
      421 ccccctccag ctactcagat atcagacctc tctaaaagga agcctaagac agaagatttt
      481 cttacctttc tctgccttcg aggttctcct gcgctgccca acagcatggt gtattttgga
      541 agctctcagg atgaggagga agtcgaggag gaagatgatg agacagaaga cgtcaaaaca
      601 gccaccaaca atgcttcatc ttcatgccag tcgaccccca ggaaaggaaa aacccacaaa
      661 catgttcaca acgggcatgt tttcaatggt tccagcaggt caacacggga gaaggaacct
      721 gttcaaaaac acaaaagcaa agaggccact cccgcaaagg agaagcacag cgatcaccgg
      781 gctgacagcc gccgggagca ggcttcagct aaccaccccg cagcggcccc ctccacgggt
      841 tcctcggcca aggggcttgc tgccacccat caccaccccc ctctgcatcg gtcggctcag
      901 gacttacgga aacaggtttc taaggtaaac ggagtcactc gaatgtcatc tctgggtgca
      961 ggtgtaacca gtgccaaaaa gatgcgcgag gtcagacctt caccatccaa aactgtgaag
     1021 tacactgcca cggtgacgaa gggggctgtc acatacacca aagccaagag agaactggtc
     1081 aaggacacca aacccaatca ccacaagccc agttccgctg tcaaccacac aatctcaggg
     1141 aaaactgaaa gtagcaatgc aaaaacccgc aaacaggtgc tatccctcgg gggggcgtcc
     1201 aagtccactg ggcccgccgt caatggcctc aaggtcagtg gcaggttgaa cccaaagtca
     1261 tgcactaagg aggtgggggg gcggcagctg cgggagggcc tgcagctgcg ggaggggctg
     1321 cggaactcca agaggagact ggaagaggca caccaggcgg agaagccgca gtcgcccccc
     1381 aagaagatga aaggggcggc tggccccgcc gaaggccctg gcaagaaggc cccggccgag
     1441 agaggtctgc tgaacggaca cgtgaagaag gaagtgccgg agcgcagtct ggagaggaat
     1501 cggccgaagc gggccacggc cgggaagagc acgccaggca gacaagcaca tggcaaggcg
     1561 gacagcgcct cctgtgaaaa tcgttctacc tcgcaaccgg agtccgtgca caagccgcag
     1621 gactcgggca aggccgagaa gggcggcggc aaggccgggt gggcggccat ggacgagatc
     1681 cccgtcctca ggccctccgc caaggagttc cacgatccgc tcatctacat cgagtcggtc
     1741 cgcgctcagg tggagaagtt cgggatgtgc agggtgatcc cccctccgga ctggcggccc
     1801 gagtgcaagc tcaacgatga gatgcggttt gtcacgcaga ttcagcacat ccacaagctg
     1861 ggccggcgct ggggccccaa cgtgcagcgg ctggcctgca tcaagaagca cctcaaatct
     1921 cagggcatca ccatggacga gctcccgctc atagggggct gtgagctcga cctggcctgc
     1981 tttttccggc tgattaatga gatgggcggc atgcagcaag tgactgacct caaaaaatgg
     2041 aacaaactag cagacatgct gcgcatcccc agaactgccc aggaccggct ggccaagctg
     2101 caggaggcct actgccagta cctactctcc tacgactccc tgtccccaga ggagcaccgg
     2161 cggctggaga aggaggtgct gatggagaag gagatcctgg agaagcgcaa ggggccgctg
     2221 gaaggccaca cagagaacga ccaccacaag ttccaccctc tgccccgctt cgagcccaag
     2281 aatgggctca tccacggcgt ggcccccagg aacggcttcc gcagcaagct caaggaggtg
     2341 ggccaggccc agttgaagac tggccggcgg cgactcttcg ctcaggaaaa agaagtggtc
     2401 aaggaagagg aggaggacaa aggcgtcctc aatgacttcc acaagtgcat ctataaggga
     2461 aggtctgttt ctctaacaac tttttatcga acagcgagga atatcatgag catgtgtttc
     2521 agcaaggagc ctgccccagc cgaaatcgag caagagtact ggaggctagt ggaagagaag
     2581 gactgccacg tggcagtgca ctgcggcaag gtggacacca acactcacgg cagtggattc
     2641 ccagtaggaa aatcagaacc cttttcgagg catggatgga acctcaccgt cctccccaat
     2701 aacacagggt ccatcctgcg tcacctcggt gctgtgcctg gagtgactat tccctggcta
     2761 aatattggca tggtcttttc tacctcatgc tggtctcgag accaaaatca ccttccatac
     2821 attgactact tacacactgg tgctgactgc atttggtatt gcattcctgc tgaggaggag
     2881 aacaagctgg aagatgtggt ccacaccctg ctgcaagcca atggcacccc agggctgcag
     2941 atgctggaaa gcaacgtcat gatctccccg gaggtgctgt gcaaagaggg gatcaaggtg
     3001 cacaggaccg tgcagcagag tggccagttt gtcgtctgct tcccgggatc ctttgtgtcc
     3061 aaagtgtgct gtgggtacag cgtgtctgaa accgtgcact ttgctaccac ccagtggaca
     3121 agtatgggct ttgagaccgc caaggaaatg aagcgtcgcc atatagctaa gccattctcc
     3181 atggagaagt tactctacca gattgcacaa gcagaagcaa aaaaagaaaa cggtcccact
     3241 ctcagtacca tctcagccct cctggatgag ctcagggata cagagctgcg gcagcgcagg
     3301 cagctgttcg aggctggcct ccactcctcc gcacgctatg gcagccacga tggcagcagc
     3361 acggtggcgg acgggaagaa aaagcctcga aagtggctgc agttggagac gtcagagagg
     3421 aggtgtcaga tctgccagca cctgtgctac ctgtccatgg tggtacaaga gaacgaaaac
     3481 gtcgtgttct gtctggagtg tgctctgcgc cacgtggaga aacagaagtc ctgccgaggg
     3541 ctgaagttga tgtaccgcta cgatgaggaa cagattatca gtctggtcaa tcagatctgc
     3601 ggcaaagtgt ctggtaaaaa cggcagcatt gagaactgtc tcagtaaacc cacaccaaaa
     3661 agaggtcccc gcaagagagc gacagtggac gtgcccccct cccgtctgtc agcctccagt
     3721 tcatccaaaa gtgcttcgag ctcatcatag taatgaacta gtccgtaact tgaaagtatt
     3781 tcgatttctt ggctttatat atcttgtgga aaggacgagt cgctaccctc gtaacggctt
     3841 gt
//
