LOCUS       sequence-279254-        1589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1320
FEATURES             Location/Qualifiers
     source          1..1589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacatatg tgagtccatc ctggagagga agcggcatga cagcgaaagg
       61 tctacttgca gcatcttgga gcagacagac atggaagctg tcgaggctct tgtttgtatg
      121 agctcctggg gtcaaagatc ccagaaaggt gacctgttgc ggataagacc cctcacgcct
      181 gtctctgact ctggggatgt caccaccact gtgcatatgg atgcagccac acctgaacta
      241 ccaaaagact tccattcttt atcgactctg tgcataactc ctcctcagag ccctgatctc
      301 gtggagccat cgacaaggac acctgtttct ccccaagtaa cagattccaa agcatgtaca
      361 gccacggatg ttctccagtc ctctgccgta gtggccagag ctctgagcgg gggcgcggag
      421 aggggcttgc tgggtttgga gccagtgccc agctctccct gcagggccaa ggggactagc
      481 gtgatccgac acactgggga gagccctgct gcctgctttc ccaccatcca gactccagat
      541 tgccggcttt ctgacagcag agaaggagaa gagcagcttc tgggacactt tgaaactttg
      601 caggacacac acctcacgga cagtttactc agcactaact tggtgtcctg tcagccctgc
      661 ttgcacaagt ctggtggcct gctgctcact gacaaaggcc agcaggcagg gtggcctggt
      721 gcagttcaga cttgctcacc aaagaattat gaaaatgacc tgcccaggaa aaccacccct
      781 ctgatttctg tctctgtccc tgctccccct gtcctttgcc agatgatccc tgtgactgga
      841 caaagtagca tgttaccagc ttttttgaag ccccctcccc agttgtctgt ggggactgtg
      901 agacccatcc tagctcaggc tgctccagcg cctcaacctg tgttcgtggg acctgctgtg
      961 cctcagggag ctgtgatgtt ggtcctgccc cagggagccc tccctccgcc tgccccctgt
     1021 gcagccaatg tcatggctgc cgggaatacc aagttgttgc cccttgcccc tgctccagtg
     1081 ttcatcacct ctagccaaaa ctgtgtccct caggtagact tttcccgaag gaggaactat
     1141 gtatgcagct tcccaggttg ccggaagacc tacttcaaaa gttcccacct taaggcccat
     1201 cttcgcactc acacagggga gaagcctttc aactgcagct gggatggctg tgataaaaag
     1261 tttgctcgtt cggatgagct gtcacgccac cgcagaactc acacagggga gaagaagttt
     1321 gtgtgcccgg tgtgtgaccg acgtttcatg cgcagtgacc acctgacgaa gcatgcccgg
     1381 cgccacatga cgaccaagaa gatcccaggc tggcaggcag aggttggcaa gctgaacaga
     1441 atcgcctctg cagagagccc ggggagccca ctggtgagca tgccagcctc tgcctagtaa
     1501 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1561 gacgatggcg gaactgccgg atgtaccac
//
