LOCUS       sequence-279257-        2315 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2655
FEATURES             Location/Qualifiers
     source          1..2315
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaacagtt ccgccacttg ccaatgcctt tccactggaa gcaggaagaa
       61 ctcaagttta agacaggctt gcggaggctg cagcaccgag taggggagat ccaccttctc
      121 cgagaagccc tgcagaaggg ggctgaggct ggccaagtgt ctctgcacag cttgatagaa
      181 actcctgcta atgggactgg gccaagtgag gccctggcca tgctactgca ggagaccact
      241 ggagagctag aggcagccaa agccctagtg ctgaagagga tccagatttg gaaacggcag
      301 cagcagctgg cagggaatgg cgcaccgttt gaggagagcc tggccccact ccaggagagg
      361 tgtgaaagcc tggtggacat ttattcccag ctacagcagg aggtaggggc ggctggtggg
      421 gagcttgagc ccaagacccg ggcatcgctg actggccggc tggatgaagt cctgagaacc
      481 ctcgtcacca gttgcttcct ggtggagaag cagccccccc aggtactgaa gactcagacc
      541 aagttccagg ctggagttcg attcctgttg ggcttgaggt tcctgggggc cccagccaag
      601 cctccgctgg tcagggccga catggtgaca gagaagcagg cgcgggagct gagtgtgcct
      661 cagggtcctg gggctggagc agaaagcact ggagaaatca tcaacaacac tgtgcccttg
      721 gagaacagca ttcctgggaa ctgctgctct gccctgttca agaacctgct tctcaagaag
      781 atcaagcggt gtgagcggaa gggcactgag tctgtcacag aggagaagtg cgctgtgctc
      841 ttctctgcca gcttcacact tggccccggc aaactcccca tccagctcca ggccctgtct
      901 ctgcccctgg tggtcatcgt ccatggcaac caagacaaca atgccaaagc cactatcctg
      961 tgggacaatg ccttctctga gatggaccgc gtgccctttg tggtggctga gcgggtgccc
     1021 tgggagaaga tgtgtgaaac tctgaacctg aagttcatgg ctgaggtggg gaccaaccgg
     1081 gggctgctcc cagagcactt cctcttcctg gcccagaaga tcttcaatga caacagcctc
     1141 agtatggagg ccttccagca ccgttctgtg tcctggtcgc agttcaacaa ggagatcctg
     1201 ctgggccgtg gcttcacctt ttggcagtgg tttgatggtg tcctggacct caccaaacgc
     1261 tgtctccgga gctactggtc tgaccggctg atcattggct tcatcagcaa acagtacgtt
     1321 actagccttc ttctcaatga gcccgacgga acctttctcc tccgcttcag cgactcagag
     1381 attgggggca tcaccattgc ccatgtcatc cggggccagg atggctctcc acagatagag
     1441 aacatccagc cattctctgc caaagacctg tccattcgct cactggggga ccgaatccgg
     1501 gatcttgctc agctcaaaaa tctctatccc aagaagccca aggatgaggc tttccggagc
     1561 cactacaagc ctgaacagat gggtaaggat ggcaggggtt atgtcccagc taccatcaag
     1621 atgaccgtgg aaagggacca accacttcct accccagagc tccagatgcc taccatggtg
     1681 ccttcttatg accttggaat ggcccctgat tcctccatga gcatgcagct tggcccagat
     1741 atggtgcccc aggtgtaccc accacactct cactccatcc ccccgtatca aggcctctcc
     1801 ccagaagaat cagtcaacgt gttgtcagcc ttccaggagc ctcacctgca gatgcccccc
     1861 agcctgggcc agatgagcct gccctttgac cagcctcacc cccagggcct gctgccgtgc
     1921 cagcctcagg agcatgctgt gtccagccct gaccccctgc tctgctcaga tgtgaccatg
     1981 gtggaagaca gctgcctgag ccagccagtg acagcgtttc ctcagggcac ttggattggt
     2041 gaagacatat tccctcctct gctgcctccc actgaacagg acctcactaa gcttctcctg
     2101 gaggggcaag gggagtcggg gggagggtcc ttgggggcac agcccctcct gcagccctcc
     2161 cactatgggc aatctgggat ctcaatgtcc cacatggacc taagggccaa ccccagttgg
     2221 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2281 ggaaaggacg atgttacgat gagcagctaa ttgag
//
