LOCUS       sequence-279275-        2741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1358
FEATURES             Location/Qualifiers
     source          1..2741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaggtgg gggcggcgac ctgagcacca ggaggctgaa tgaatgtatt
       61 tcaccagtag caaatgagat gaaccatctt cctgcacaca gccacgattt gcaaaggatg
      121 ttcacggaag accagggtgt agatgacagg ctgctctatg acattgtatt caagcacttc
      181 aaaagaaata aggtggagat ttcaaatgca ataaaaaaga catttccatt cctcgagggc
      241 ctccgtgatc gtgatctcat cacaaataaa atgtttgaag attctcaaga ttcttgtaga
      301 aacctggtcc ctgtacagag agtggtgtac aatgttctta gtgaactgga gaagacattt
      361 aacctgccag ttctggaagc actgttcagc gatgtcaaca tgcaggaata ccccgattta
      421 attcacattt ataaaggctt tgaaaatgta atccatgaca aattgcctct ccaagaaagt
      481 gaagaagaag agagggagga gaggtctggc ctccaactaa gtcttgaaca aggaactggt
      541 gaaaactctt ttcgaagcct gacttggcca ccttcgggtt ccccatctca tgctggtaca
      601 accccacctg aaaatggact ctcagagcac ccctgtgaaa cagaacagat aaatgcaaag
      661 agaaaagata caaccagtga caaagatgat tcgctaggaa gccaacaaac aaatgaacaa
      721 tgtgctcaaa aggctgagcc aacagagtcc tgcgaacaaa ttgctgtcca agtgaataat
      781 ggggatgctg gaagggagat gccctgcccg ttgccctgtg atgaagaaag cccagaggca
      841 gagctacaca accatggaat ccaaattaat tcctgttctg tgcgactggt ggatataaaa
      901 aaggaaaagc cattttctaa ttcaaaagtt gagtgccaag cccaagcaag aactcatcat
      961 aaccaggcat ctgacataat agtcatcagc agtgaggact ctgaaggatc cactgacgtt
     1021 gatgagccct tagaagtctt catctcagca ccgagaagtg agcctgtgat caataatgac
     1081 aaccctttag aatcaaatga tgaaaaggag ggccaagaag ccacttgctc acgaccccag
     1141 attgtaccag agcccatgga tttcagaaaa ttatctacat tcagagaaag ttttaagaaa
     1201 agagtgatag gacaagacca cgacttttca gaatccagtg aggaggaggc gcccgcagaa
     1261 gcctcgagcg gggcactgag aagcaagcat ggtgagaagg ctcctatgac ttctagaagt
     1321 acatctactt ggagaatacc cagcaggaag agacgtttca gcagtagtga cttttcagac
     1381 ctgagtaatg gagaagagct tcaggaaacc tgcagctcat ccctaagaag agggtcagga
     1441 tcacagccac aagaacctga aaataagaag tgctcctgtg tcatgtgttt tccaaaaggt
     1501 gtgccaagaa gccaagaagc aaggactgaa agtagtcaag catctgacat gatggatacc
     1561 atggatgttg aaaacaattc tactttggaa aaacacagtg ggaaaagaag aaaaaagaga
     1621 aggcatagat ctaaagtaaa tggtctccaa agagggagaa agaaagacag acctagaaaa
     1681 catttaactc tgaataacaa agtccaaaag aaaagatggc aacaaagagg aagaaaagcc
     1741 aacactagac ctttgaaaag aagaagaaaa agaggtccaa gaattcccaa agatgaaaat
     1801 attaatttta aacaatctga acttcctgtg acctgtggtg aggtgaaggg cactctatat
     1861 aaggagcgat tcaaacaagg aacctcaaag aagtgtatac agagtgagga taaaaagtgg
     1921 ttcactccca gggaatttga aattgaagga gaccgcggag catccaagaa ctggaagcta
     1981 agtatacgct gcggtggata taccctgaaa gtcctgatgg agaacaaatt tctgccagaa
     2041 ccaccaagca caagaaaaaa gagaatactg gaatctcaca acaatacctt agttgaccct
     2101 tgtgaggagc ataagaagaa gaacccagat gcttcagtca agttctcaga gtttttaaag
     2161 aagtgctcag agacatggaa gaccattttt gctaaagaga aaggaaaatt tgaagatatg
     2221 gcaaaggcgg acaaggccca ttatgaaaga gaaatgaaaa cctatatccc tcctaaaggg
     2281 gagaaaaaaa agaagttcaa ggatcccaat gcacccaaga ggcctccttt ggcctttttc
     2341 ctgttctgct ctgagtatcg cccaaaaatc aaaggagaac atcctggcct gtccattgat
     2401 gatgttgtga agaaactggc agggatgtgg aataacaccg ctgcagctga caagcagttt
     2461 tatgaaaaga aggctgcaaa gctgaaggaa aaatacaaaa aggatattgc tgcatatcga
     2521 gctaaaggaa agcctaattc agcaaaaaag agagttgtca aggctgaaaa aagcaagaaa
     2581 aagaaggaag aggaagaaga tgaagaggat gaacaagagg aggaaaatga agaagatgat
     2641 gataaatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2701 tcttgtggaa aggacgagaa gtacatactg atgtgatagg c
//
