LOCUS       sequence-279287-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1376
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             266..274
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgtcacacct cagccagcag agaatttaca gtggggaaaa cccctttgcc
       61 tgtaaggtat gtggaaaagt cttcagccac aaatcaaacc tcactgagca tgagcatttt
      121 cacacgagag agaaaccttt tgaatgtaac gagtgtggaa aagcctttag ccaaaagcag
      181 tatgtcatta aacatcagaa cacccatact ggcgagaagc ttttcgaatg taatgaatgt
      241 ggaaaatcat ttagccagaa ggaaaacctc cttacgcacc agaaaattca cactggagaa
      301 aaaccttttg agtgtaaaga ttgcgggaaa gctttcattc agaagtcaaa cctcatcaga
      361 caccagagaa ctcacacagg agagaagccc tttgtatgta aggagtgtgg aaaaaccttc
      421 agtggcaaat ccaaccttac tgagcatgag aaaatccata ttggagagaa gccttttaaa
      481 tgtagtgaat gtggaacagc ctttggccag aagaagtacc tcataaaaca tcagaacatt
      541 cacactggag agaaacccta tgaatgtaac gaatgtggaa aagccttctc tcagcgaaca
      601 tcacttattg tacatgtgag gattcattca ggtgataaac cttacgaatg caatgtttgt
      661 ggaaaagcct tctctcagag ctcatctctc actgtgcatg tgagaagcca tacaggggag
      721 aagccctatg gttgtaatga atgtgggaaa gctttctctc agttctcaac ccttgctctg
      781 catttgagaa tacacacagg taagaagcct tatcagtgca gtgaatgtgg gaaagctttc
      841 agccagaagt cacaccacat tagacaccag aaaattcata ctcactagta atgaactagt
      901 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgagatt
      961 tcggaggatc ctctgtgtga
//
