LOCUS       sequence-27929-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Todi SV, Laco MN, Winborn BJ, Travis SM, Wen HM, Paulson HL
  TITLE     Cellular turnover of the polyglutamine disease protein ataxin-3 is 
            regulated by its catalytic activity.
  JOURNAL   J Biol Chem. 2007 Oct 5. 282(40):29348-58.
  PUBMED    17693639
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2007 Oct 5. 282(40):29348-58."
COMMENT     Sequence Label: pVETL-Cmcs-Flag-Ataxin3Q80-C14A
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(538..561)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     gap             666..678
                     /estimated_length=13
     primer_bind     complement(693..713)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gcttcgcaat ctggcagatc acccttaaca acaaatatag aataaccttc ctgnttgtaa
       61 ttgagccaag aaaagtgcaa gatatgtatc tgatattaat tctggacccg tcaagagaga
      121 attcaagtta aaccactgtt ttcctaattt tctaactgta aaccagtgtt ccttataatt
      181 gcatataaat gatctttcat ttataggatc gatcctgagc ctctgatact ctggactgtt
      241 gaacaggatt agttctaaac cccaaacttt caaggcattg cttataacct gaatagagaa
      301 aaaaccactg tcatccatat ttccagaagg ctgctgtaaa aacgtgcgat aatcttcact
      361 agtaactcct ccttctgcca ttctcatcct ctcctcctca tccagctgat gtgcaattga
      421 ggataattcc acagggctaa aatattctcc ttgcaataag ttattcaggc aatgttgagc
      481 agcaagtgag ccttcttgtt tctcgtggaa gatggactct ccgtttataa gcttgagctt
      541 gtcgtcatcg tctttgtagt ccatagctct agagtccgga ggctggatcg gtcccggtgt
      601 cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcctaacn
      661 gncagnnnnn nnnnnnnnaa cctcccaccg tacacgccta ccgcccattt gcgtcaatgg
      721 ggcggagttg ttacgacatt ttggaaagtc cgttgatttg gtgccaacaa ctccatgcgt
      781 catggggtgg gactggaatc ccgnagcaac ccttccnccc t
//
