LOCUS       sequence-279326-        2450 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2527
FEATURES             Location/Qualifiers
     source          1..2450
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1893..1902
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcaaaag agaccgagcg gaccgcgaca agaagaaatc caggaagcgg
       61 cactatgagg atgaagagga tgatgaagag gacgccccgg ggaacgaccc tcaggaagcg
      121 gttccctcgg cggcggggaa gcaggtggat gagtcaggca ccaaagtgga tgaatatgga
      181 gccaaggact acaggctgca aatgccgctg aaggacgacc acacctccag gcccctctgg
      241 gtggctcccg atggccatat cttcttggaa gccttctctc cagtttacaa atatgcccaa
      301 gacttcttgg tggctattgc agagccagtg tgccgaccaa cccatgtgca tgagtacaaa
      361 ctaactgcct actccttgta tgcagctgtc agcgttgggc tgcaaaccag tgacatcacc
      421 gagtacctca ggaagctcag caagactgga gtccctgatg gaattatgca gtttattaag
      481 ttgtgtactg tcagctatgg aaaagtcaag ctggtcttga agcacaacag atacttcgtt
      541 gaaagttgcc accctgatgt aatccagcat cttctccagg accccgtgat ccgagaatgc
      601 cgcttaagaa actctgaagg ggaggccact gagctcatca cagagacttt cacaagcaaa
      661 tctgccattt ctaagactgc tgaaagcagt ggtgggccct ccacttcccg agtgacagat
      721 ccacagggta aatctgacat ccccatggac ctgtttgact tctatgagca aatggacaag
      781 gatgaagaag aagaagaaga gacacagaca gtgtcttttg aagtcaagca ggaaatgatt
      841 gaggaactcc agaaacgttg catccacctg gagtaccctc tgttggcaga atatgacttc
      901 cggaatgatt ctgtcaaccc tgatatcaac attgacctaa agcccacagc tgtcctcaga
      961 ccctatcagg agaagagctt gcgaaagatg tttggaaacg ggcgtgcacg ttcgggggtc
     1021 attgttcttc cctgcggtgc tggaaagtcc ctggttggtg tgactgctgc atgcactgtc
     1081 agaaaacgct gtctggtgct gggcaactca gctgtttctg tggagcagtg gaaagcccag
     1141 ttcaagatgt ggtccaccat tgacgacagc cagatctgcc ggttcacctc cgatgccaag
     1201 gacaagccca tcggctgctc cgttgccatt agcacctact ccatgctggg ccacaccacc
     1261 aaaaggtcct gggaggccga gcgagtcatg gagtggctca agacccagga gtggggcctc
     1321 atgatcctgg atgaagtgca caccatacca gccaagatgt tccgaagggt gctcaccatc
     1381 gtgcaggccc actgtaagct gggtttgact gcgaccctcg tccgcgaaga tgacaaaatt
     1441 gtggatttaa attttctgat tgggcctaag ctctacgaag ccaactggat ggagctgcag
     1501 aataatggct acatcgccaa agtccagtgt gctgaggtct ggtgccctat gtctcctgaa
     1561 ttttaccggg aatatgtggc aatcaaaacc aagaaacgaa tcttgctgta caccatgaac
     1621 cccaacaaat ttagagcttg ccagtttctg atcaagtttc atgaaaggag gaatgacaag
     1681 attattgtct ttgctgacaa tgtgtttgcc ctaaaggaat atgccattcg actgaacaaa
     1741 ccctatatct acggacctac gtctcagggg gaaaggatgc aaattctcca gaatttcaag
     1801 cacaacccca aaattaacac catcttcata tccaaggtag gtgacacttc gtttgatctg
     1861 ccggaagcaa atgtcctcat tcagatctca tcccatggtg gctccaggcg tcaggaagcc
     1921 caaaggctag ggcgggtgct tcgagctaaa aaagggatgg ttgcagaaga gtacaatgcc
     1981 tttttctact cactggtatc ccaggacaca caggaaatgg cttactcaac caagcggcag
     2041 agattcttgg tagatcaagg ttatagcttc aaggtgatca cgaaactcgc tggcatggag
     2101 gaggaagact tggcgttttc gacaaaagaa gagcaacagc agctcttaca gaaagtcctg
     2161 gcagccactg acctggatgc cgaggaggag gtggtggctg gggaatttgg ctccagatcc
     2221 agccaggcat ctcggcgctt tggcaccatg agttctatgt ctggggccga cgacactgtg
     2281 tacatggagt accactcatc gcggagcaag gcgcccagca aacatgtaca cccgctcttc
     2341 aagcgcttta ggaaatagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2401 ctttatatat cttgtggaaa ggacgactgg ctagttccat gatattacca
//
