LOCUS       sequence-279347-        1949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2301
FEATURES             Location/Qualifiers
     source          1..1949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgtctgg gcccttggca ctccggtggt gcgcgtgggc agggcgcggg
       61 gacatggggc ccgacatgga gctgcccagc cactcgaagc agctcctgct gcagctgaac
      121 cagcagagga ccaagggctt cctgtgtgac gtcatcatca tggtggagaa ctccatcttc
      181 cgggcccaca agaacgtcct agccgccagc agcatctatt tcaagtccct ggtcctgcac
      241 gacaacctca tcaacctgga cacagacatg gtcagctcca cagtgttcca gcagatcttg
      301 gacttcatct acacaggcaa gctgctgccc agcgaccagc cagccgagcc caacttcagc
      361 accctcctca ctgccgccag ctacctccag ctgcccgagt tggcagccct ctgccgccgc
      421 aaactcaagc gagccggcaa gccctttggc tctgggaggg cggggtccac tggcatgggg
      481 cggccccccc gcagccagcg gctgtccacg gcctctgtca tccaagctcg gtatcagggg
      541 ctcgtggatg ggcgcaaggg ggcccacgcc ccccaggagc tcccccaagc caaaggctca
      601 gacgatgaac tctttcttgg tggctctaac caggatagcg tgcaaggtct gggccgggct
      661 gtctgcccag ctggcgggga ggcgggtctg gggggctgca gcagcagcac caacgggagc
      721 agcgggggct gcgagcagga gctgggcttg gacctgtcca agaaaagccc acccttgccc
      781 cctgccaccc caggtcccca cctcactccc gatgacgcag cccagctgag cgacagccaa
      841 catggctcgc cccctgcggc ctctgctcct cccgttgcca acagtgcctc ttattctgag
      901 ctggggggca cccctgatga gcccatggat ctggaggggg ccgaggacaa ccacctgagc
      961 ctgctggagg cgcctggtgg gcagcctcgg aagagcctcc ggcactccac tcggaagaag
     1021 gagtggggca agaaggagcc tgtggctggc tccccctttg agcggagaga agcagggccc
     1081 aagggtccct gcccgggaga ggagggtgag ggggtcgggg acagggttcc caatggcatc
     1141 ctggctagtg gggctggccc tagcgggccc tatggggagc ccccctaccc ctgcaaggag
     1201 gaggaggaga acggcaagga tgcaagtgaa gacagtgcgc agagcgggag cgaggggggc
     1261 agcggccatg ccagcgccca ctacatgtac cggcaggagg gctacgagac ggtgtcctac
     1321 ggggacaact tgtatgtgtg cattccctgc gccaagggct tccccagctc tgagcagctc
     1381 aatgcgcacg tggagactca cacggaggaa gagctgttca tcaaggaaga gggggcctac
     1441 gagacaggca gtgggggtgc cgaggaggag gccgaggacc tgtcagcacc cagtgcggcc
     1501 tacacggctg agccccggcc cttcaagtgt tcggtctgcg agaagaccta caaggaccca
     1561 gccacgctgc ggcagcacga gaagacgcac tggctgacac ggcccttccc ctgcaacatc
     1621 tgtggcaaaa tgttcacgca gcgcggcacc atgacgcgtc acatgcggag ccacctgggc
     1681 ctgaagccct tcgcctgcga tgagtgtggc atgcgcttca cccgtcagta ccgcctcacg
     1741 gagcacatgc gtgtgcactc gggcgagaaa ccttacgagt gccagctgtg cgggggcaag
     1801 ttcacccagc agcgcaacct catcagccac ctgcgcatgc acacctcccc ctcctagtaa
     1861 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1921 gacgaaatgg aataataatc ggcgacgca
//
