LOCUS       sequence-279377-        1850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2402
FEATURES             Location/Qualifiers
     source          1..1850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatttgat tgacatactt tggaggcaag atatagatct tggagtaagt
       61 cgagaagtat ttgacttcag tcagcgacgg aaagagtatg agctggaaaa acagaaaaaa
      121 cttgaaaagg aaagacaaga acaactccaa aaggagcaag agaaagcctt tttcgctcag
      181 ttacaactag atgaagagac aggtgaattt ctcccaattc agccagccca gcacatccag
      241 tcagaaacca gtggatctgc caactactcc caggttgccc acattcccaa atcagatgct
      301 ttgtactttg atgactgcat gcagcttttg gcgcagacat tcccgtttgt agatgacaat
      361 gagtcacttg ttcctgatat tcccggtcac atcgagagcc cagtcttcat tgctactaat
      421 caggctcagt cacctgaaac ttctgttgct caggtagccc ctgttgattt agacggtatg
      481 caacaggaca ttgagcaagt ttgggaggag ctattatcca ttcctgagtt acagtgtctt
      541 aatattgaaa atgacaagct ggttgagact accatggttc caagtccaga agccaaactg
      601 acagaagttg acaattatca tttttactca tctataccct caatggaaaa agaagtaggt
      661 aactgtagtc cacattttct taatgctttt gaggattcct tcagcagcat cctctccaca
      721 gaagacccca accagttgac agtgaactca ttaaattcag atgccacagt caacacagat
      781 tttggtgatg aattttattc tgctttcata gctgagccca gtatcagcaa cagcatgccc
      841 tcacctgcta ctttaagcca ttcactctct gaacttctaa atgggcccat tgatgtttct
      901 gatctatcac tttgcaaagc tttcaaccaa aaccaccctg aaagcacagc agaattcaat
      961 gattctgact ccggcatttc actaaacaca agtcccagtg tggcatcacc agaacactca
     1021 gtggaatctt ccagctatgg agacacacta cttggcctca gtgattctga agtggaagag
     1081 ctagatagtg cccctggaag tgtcaaacag aatggtccta aaacaccagt acattcttct
     1141 ggggatatgg tacaaccctt gtcaccatct caggggcaga gcactcacgt gcatgatgcc
     1201 caatgtgaga acacaccaga gaaagaattg cctgtaagtc ctggtcatcg gaaaacccca
     1261 ttcacaaaag acaaacattc aagccgcttg gaggctcatc tcacaagaga tgaacttagg
     1321 gcaaaagctc tccatatccc attccctgta gaaaaaatca ttaacctccc tgttgttgac
     1381 ttcaacgaaa tgatgtccaa agagcagttc aatgaagctc aacttgcatt aattcgggat
     1441 atacgtagga ggggtaagaa taaagtggct gctcagaatt gcagaaaaag aaaactggaa
     1501 aatatagtag aactagagca agatttagat catttgaaag atgaaaaaga aaaattgctc
     1561 aaagaaaaag gagaaaatga caaaagcctt cacctactga aaaaacaact cagcacctta
     1621 tatctcgaag ttttcagcat gctacgtgat gaagatggaa aaccttattc tcctagtgaa
     1681 tactccctgc agcaaacaag agatggcaat gttttccttg ttcccaaaag taagaagcca
     1741 gatgttaaga aaaactagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1801 ctttatatat cttgtggaaa ggacgagatg tgtgaaagat gctatcgagg
//
