LOCUS       sequence-279380-        1871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2403
FEATURES             Location/Qualifiers
     source          1..1871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             1847..1855
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tggatttgat tgacatactt tggaggcaag atatagatct tggagtaagt
       61 cgagaagtat ttgacttcag tcagcgacgg aaagagtatg agctggaaaa acagaaaaaa
      121 cttgaaaagg aaagacaaga acaactccaa aaggagcaag agaaagcctt tttcgctcag
      181 ttacaactag atgaagagac aggtgaattt ctcccaattc agccagccca gcacatccag
      241 tcagaaacca gtggatctgc caactactcc caggttgccc acattcccaa atcagatgct
      301 ttgtactttg atgactgcat gcagcttttg gcgcagacat tcccgtttgt agatgacaat
      361 gaggtttctt cggctacgtt tcagtcactt gttcctgata ttcccggtca catcgagagc
      421 ccagtcttca ttgctactaa tcaggctcag tcacctgaaa cttctgttgc tcaggtagcc
      481 cctgttgatt tagacggtat gcaacaggac attgagcaag tttgggagga gctattatcc
      541 attcctgagt tacagtgtct taatattgaa aatgacaagc tggttgagac taccatggtt
      601 ccaagtccag aagccaaact gacagaagtt gacaattatc atttttactc atctataccc
      661 tcaatggaaa aagaagtagg taactgtagt ccacattttc ttaatgcttt tgaggattcc
      721 ttcagcagca tcctctccac agaagacccc aaccagttga cagtgaactc attaaattca
      781 gatgccacag tcaacacaga ttttggtgat gaattttatt ctgctttcat agctgagccc
      841 agtatcagca acagcatgcc ctcacctgct actttaagcc attcactctc tgaacttcta
      901 aatgggccca ttgatgtttc tgatctatca ctttgcaaag ctttcaacca aaaccaccct
      961 gaaagcacag cagaattcaa tgattctgac tccggcattt cactaaacac aagtcccagt
     1021 gtggcatcac cagaacactc agtggaatct tccagctatg gagacacact acttggcctc
     1081 agtgattctg aagtggaaga gctagatagt gcccctggaa gtgtcaaaca gaatggtcct
     1141 aaaacaccag tacattcttc tggggatatg gtacaaccct tgtcaccatc tcaggggcag
     1201 agcactcacg tgcatgatgc ccaatgtgag aacacaccag agaaagaatt gcctgtaagt
     1261 cctggtcatc ggaaaacccc attcacaaaa gacaaacatt caagccgctt ggaggctcat
     1321 ctcacaagag atgaacttag ggcaaaagct ctccatatcc cattccctgt agaaaaaatc
     1381 attaacctcc ctgttgttga cttcaacgaa atgatgtcca aagagcagtt caatgaagct
     1441 caacttgcat taattcggga tatacgtagg aggggtaaga ataaagtggc tgctcagaat
     1501 tgcagaaaaa gaaaactgga aaatatagta gaactagagc aagatttaga tcatttgaaa
     1561 gatgaaaaag aaaaattgct caaagaaaaa ggagaaaatg acaaaagcct tcacctactg
     1621 aaaaaacaac tcagcacctt atatctcgaa gttttcagca tgctacgtga tgaagatgga
     1681 aaaccttatt ctcctagtga atactccctg cagcaaacaa gagatggcaa tgttttcctt
     1741 gttcccaaaa gtaagaagcc agatgttaag aaaaactagt aatgaactag tccgtaactt
     1801 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacct ccttagtgtc
     1861 ttgagtatat g
//
