LOCUS       sequence-279413-        3551 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2443
FEATURES             Location/Qualifiers
     source          1..3551
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggcagca gcctggaaaa gtacttgggg accaaagaag gccaagcttg
       61 cctgccctgc attttatcaa aggagcaggg aagaaggaat catcgaggca tgggggtcca
      121 cactgcaatg tttttgtgga acatgaagcc cttcagcggc cagtagcatc tgactttgag
      181 cctcagggtc tgagtgaagc cgctcgttgg aactccaagg aaaaccttct cgctggaccc
      241 agtgaaaatg accccaacct tttcgttgca ctgtatgatt ttgtggccag tggagataac
      301 actctaagca taactaaagg tgaaaagctc cgggtcttag gctataatca caatggggaa
      361 tggtgtgaag cccaaaccaa aaatggccaa ggctgggtcc caagcaacta catcacgcca
      421 gtcaacagtc tggagaaaca ctcctggtac catgggcctg tgtcccgcaa tgccgctgag
      481 tatctgctga gcagcgggat caatggcagc ttcttggtgc gtgagagtga gagcagtcct
      541 ggccagaggt ccatctcgct gagatacgaa gggagggtgt accattacag gatcaacact
      601 gcttctgatg gcaagctcta cgtctcctcc gagagccgct tcaacaccct ggccgagttg
      661 gttcatcatc attcaacggt ggccgacggg ctcatcacca cgctccatta tccagcccca
      721 aagcgcaaca agcccactgt ctatggtgtg tcccccaact acgacaagtg ggagatggaa
      781 cgcacggaca tcaccatgaa gcacaagctg ggcgggggcc agtacgggga ggtgtacgag
      841 ggcgtgtgga agaaatacag cctgacggtg gccgtgaaga ccttgaagga ggacaccatg
      901 gaggtggaag agttcttgaa agaagctgca gtcatgaaag agatcaaaca ccctaacctg
      961 gtgcagctcc ttggggtctg cacccgggag cccccgttct atatcatcac tgagttcatg
     1021 acctacggga acctcctgga ctacctgagg gagtgcaacc ggcaggaggt gaacgccgtg
     1081 gtgctgctgt acatggccac tcagatctcg tcagccatgg agtacctgga gaagaaaaac
     1141 ttcatccaca gagatcttgc tgcccgaaac tgcctggtag gggagaacca cttggtgaag
     1201 gtagctgatt ttggcctgag caggttgatg acaggggaca cctacacagc ccatgctgga
     1261 gccaagttcc ccatcaaatg gactgcaccc gagagcctgg cctacaacaa gttctccatc
     1321 aagtccgacg tctgggcatt tggagtattg ctttgggaaa ttgctaccta tggcatgtcc
     1381 ccttacccgg gaattgacct gtcccaggtg tatgagctgc tagagaagga ctaccgcatg
     1441 gagcgcccag aaggctgccc agagaaggtc tatgaactca tgcgagcatg ttggcagtgg
     1501 aatccctctg accggccctc ctttgctgaa atccaccaag cctttgaaac aatgttccag
     1561 gaatccagta tctcagacga agtggaaaag gagctgggga aacaaggcgt ccgtggggct
     1621 gtgagtacct tgctgcaggc cccagagctg cccaccaaga cgaggacctc caggagagct
     1681 gcagagcaca gagacaccac tgacgtgcct gagatgcctc actccaaggg ccagggagag
     1741 agcgatcctc tggaccatga gcctgccgtg tctccattgc tccctcgaaa agagcgaggt
     1801 cccccggagg gcggcctgaa tgaagatgag cgccttctcc ccaaagacaa aaagaccaac
     1861 ttgttcagcg ccttgatcaa gaagaagaag aagacagccc caacccctcc caaacgcagc
     1921 agctccttcc gggagatgga cggccagccg gagcgcagag gggccggcga ggaagagggc
     1981 cgagacatca gcaacggggc actggctttc acccccttgg acacagctga cccagccaag
     2041 tccccaaagc ccagcaatgg ggctggggtc cccaatggag ccctccggga gtccgggggc
     2101 tcaggcttcc ggtctcccca cctgtggaag aagtccagca cgctgaccag cagccgccta
     2161 gccaccggcg aggaggaggg cggtggcagc tccagcaagc gcttcctgcg ctcttgctcc
     2221 gcctcctgcg ttccccatgg ggccaaggac acggagtgga ggtcagtcac gctgcctcgg
     2281 gacttgcagt ccacgggaag acagtttgac tcgtccacat ttggagggca caaaagtgag
     2341 aagccggctc tgcctcggaa gagggcaggg gagaacaggt ctgaccaggt gacccgaggc
     2401 acagtaacgc ctccccccag gctggtgaaa aagaatgagg aagctgctga tgaggtcttc
     2461 aaagacatca tggagtccag cccgggctcc agcccgccca acctgactcc aaaacccctc
     2521 cggcggcagg tcaccgtggc ccctgcctcg ggcctccccc acaaggaaga agctggaaag
     2581 ggcagtgcct tagggacccc tgctgcagct gagccagtga cccccaccag caaagcaggc
     2641 tcaggtgcac cagggggcac cagcaagggc cccgccgagg agtccagagt gaggaggcac
     2701 aagcactcct ctgagtcgcc agggagggac aaggggaaat tgtccaggct caaacctgcc
     2761 ccgccgcccc caccagcagc ctctgcaggg aaggctggag gaaagccctc gcagagcccg
     2821 agccaggagg cggccgggga ggcagtcctg ggcgcaaaga caaaagccac gagtctggtt
     2881 gatgctgtga acagtgacgc tgccaagccc agccagccgg gagagggcct caaaaagccc
     2941 gtgctcccgg ccactccaaa gccacagtcc gccaagccgt cggggacccc catcagccca
     3001 gcccccgttc cctccacgtt gccatcagca tcctcggccc tggcagggga ccagccgtct
     3061 tccaccgcct tcatccctct catatcaacc cgagtgtctc ttcggaaaac ccgccagcct
     3121 ccagagcgga tcgccagcgg cgccatcacc aagggcgtgg tcctggacag caccgaggcg
     3181 ctgtgcctcg ccatctctag gaactccgag cagatggcca gccacagcgc agtgctggag
     3241 gccggcaaaa acctctacac gttctgcgtg agctatgtgg attccatcca gcaaatgagg
     3301 aacaagtttg ccttccgaga ggccatcaac aaactggaga ataatctccg ggagcttcag
     3361 atctgcccgg cgacagcagg cagtggtcca gcggccactc aggacttcag caagctcctc
     3421 agttcggtga aggaaatcag tgacatagtg cagaggtagt aatgaactag tccgtaactt
     3481 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatcg gatatgtctt
     3541 tgtgccatct a
//
