LOCUS       sequence-279422-        3185 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2451
FEATURES             Location/Qualifiers
     source          1..3185
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtctctag cgaccggccc gtgtcactgg aggacgaggt ctcccatagt
       61 atgaaggaga tgattggagg ctgttgcgtt tgctcagacg agagaggctg ggccgagaac
      121 ccgctggttt attgcgacgg gcacggctgc agcgtcgcgg tgcatcaagc ttgctatggc
      181 attgttcaag tacccactgg accgtggttt tgcaggaaat gtgaatctca ggagagagca
      241 gccagagtga gatgtgaact ttgtccccat aaggatggag ctttaaaaag aacagataat
      301 gggggttggg cccatgtggt ttgtgccctg tatattccag aggtacaatt tgccaatgtt
      361 tccacaatgg aaccaattgt tttacagtct gttccgcatg atcgttataa taagacttgc
      421 tacatttgtg atgaacaagg aagagaaagc aaagcagcca ctggtgcttg catgacatgt
      481 aataaacatg gatgtcgaca ggctttccat gtaacatgcg ctcagtttgc cggactgctt
      541 tgtgaagaag aaggtaatgg tgccgataat gtccaatact gtggctactg taaataccat
      601 tttagtaagc tgaaaaagag caaacgggga tctaataggt catatgatca aagtttaagt
      661 gattcttcct ctcactctca ggataaacat catgagaaag agaaaaaaaa atataaagag
      721 aaggacaaac acaaacagaa acacaagaag cagccagaac catcacctgc attggttcca
      781 tccttgactg ttactacaga aaaaacttat acaagcacta gcaacaactc tatatctgga
      841 tcattgaagc gcttggaaga tactactgca cgatttacaa atgcaaattt ccaggaagtc
      901 tctgcacaca cctctagtgg aaaagatgtt tcagagacta gagggtcaga gggcaaaggg
      961 aagaaatctt cagctcacag ctcaggtcaa aggggaagaa agcctggtgg tggaagaaat
     1021 ccaggaacaa ctgtgtcagc agctagccct tttcctcaag gcagtttttc aggaactcca
     1081 ggcagtgtaa agtcatcttc tggaagttca gtgcagtctc cccaggattt cctgagcttt
     1141 acagactcag atctgcgtaa tgacagttac tctcactccc aacagtcatc agcaaccaaa
     1201 gatgtacata aaggagagtc tggaagccag gaaggggggg taaatagttt tagtacctta
     1261 attggcctcc cttcaacctc agctgttact tcacagccta aaagctttga aaattcacct
     1321 ggagatttgg gtaattccag ccttcctaca gcaggatata agcgggctca aacttctggc
     1381 atagaagaag aaactgtaaa ggaaaagaaa aggaaaggaa ataaacaaag taagcatggg
     1441 cctggcagac ccaaaggaaa caaaaatcaa gagaatgttt ctcatctctc agtttcttct
     1501 gcttcaccaa catcatctgt agcatcagct gcaggaagca taacaagctc tagtctgcag
     1561 aaatctccta cattgctcag gaatggaagt ttacagagcc tcagtgttgg ctcatctcca
     1621 gttggttcag aaatttccat gcagtatcgg catgatggag cttgcccaac aactacgttc
     1681 tcagagttgc tgaatgcaat acacaacgac agaggtgaca gttctacact aacaaagcaa
     1741 gaacttaaat tcataggtat ttataacagc aatgatgtag cagtatcgtt tccaaatgta
     1801 gtatctggct cgggatctag tactcctgtc tccagctctc acttacctca gcagtcttct
     1861 gggcatttgc aacaagtagg agcgctctct ccctcagctg tgtcatctgc agcccctgct
     1921 gttgctacaa ctcaggcaaa tactctatct ggatcttctc tcagtcaggc accatctcat
     1981 atgtatggca atagatcaaa ttcatcaatg gcagctctta tagctcagtc tgaaaacaat
     2041 caaacagatc aagatcttgg agacaatagc cgcaacctag ttggcagagg aagctcaccc
     2101 cgaggaagtc tctcgccacg atcccctgta agcagcttac agattcgcta tgatcaacca
     2161 ggcaacagca gtttggaaaa tctgcctcca gtagcagcca gcatagaaca gcttttggag
     2221 aggcagtgga gtgaaggaca gcaattttta ctagaacagg gtactcctag tgacatttta
     2281 ggaatgctga agtcattaca ccaacttcaa gttgaaaacc gaagattaga ggaacaaatt
     2341 aaaaacttga ctgccaaaaa ggaacggctt cagttattga atgcacagct ttcagtgcct
     2401 tttccaacaa taacagcaaa tcctagtccg tctcatcaaa tacacacatt ttcagcacag
     2461 actgctccta ctactgattc cttgaacagc agtaagagcc ctcatatagg aaacagcttt
     2521 ttacctgata attctcttcc tgtattaaat caggacttaa cctccagtgg acaaagtacc
     2581 agcagctcat cagctctttc taccccacct cctgctgggc agagtccggc tcaacaaggc
     2641 tcaggagtga gtggagttca gcaggtcaat ggcgtgacag tgggggcact agctagtgga
     2701 atgcagcctg taacttccac cattcctgcc gtgtctgcag tgggtggaat aattggagct
     2761 ttgccaggta accaactggc aattaatggc attgtaggag ctttaaatgg ggttatgcag
     2821 actcctgtca caatgtccca gaaccctacc cctctcaccc acacaaccgt accacctaat
     2881 gcaacacatc caatgccagc tacactgact aacagtgcct caggactagg attactttct
     2941 gaccagcaac gacaaatact tattcatcaa cagcagtttc agcagttgtt aaattctcaa
     3001 cagctcacac cagtacacag gcacccccac ttcacacagc taccaccaac ccatttctca
     3061 ccatccatgg agataatgca agtcagaaag tagtaatgaa ctagtccgta acttgaaagt
     3121 atttcgattt cttggcttta tatatcttgt ggaaaggacg atgtccgggc aagaatacga
     3181 gttga
//
