LOCUS       sequence-279437-        2750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2501
FEATURES             Location/Qualifiers
     source          1..2750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcctgc acccgcccga tctccgaggc cccagcagga ccccgcccgg
       61 ccccaggagc ccaccatgcc tccccccgag accccctctg aaggccgcca gcccagcccc
      121 agccccagcc ctacagagcg agcccccgct tcggaggagg agttccagtt tctgcgctgc
      181 cagcaatgcc aggcggaagc caagtgcccg aagctgctgc cttgtctgca cacgctgtgc
      241 tcaggatgcc tggaggcgtc gggcatgcag tgccccatct gccaggcgcc ctggccccta
      301 ggtgcagaca cacccgccct ggataacgtc tttttcgaga gtctgcagcg gcgcctgtcg
      361 gtgtaccggc agattgtgga tgcgcaggct gtgtgcaccc gctgcaaaga gtcggccgac
      421 ttctggtgct ttgagtgcga gcagctcctc tgcgccaagt gcttcgaggc acaccagtgg
      481 ttcctcaagc acgaggcccg gcccctagca gagctgcgca accagtcggt gcgtgagttc
      541 ctggacggca cccgcaagac caacaacatc ttctgctcca accccaacca ccgcacccct
      601 acgctgacca gcatctactg ccgaggatgt tccaagccgc tgtgctgctc gtgcgcgctc
      661 cttgacagca gccacagtga gctcaagtgc gacatcagcg cagagatcca gcagcgacag
      721 gaggagctgg acgccatgac gcaggcgctg caggagcagg atagtgcctt tggcgcggtt
      781 cacgcgcaga tgcacgcggc cgtcggccag ctgggccgcg cgcgtgccga gaccgaggag
      841 ctgatccgcg agcgcgtgcg ccaggtggta gctcacgtgc gggctcagga gcgcgagctg
      901 ctggaggctg tggacgcgcg gtaccagcgc gactacgagg agatggccag tcggctgggc
      961 cgcctggatg ctgtgctgca gcgcatccgc acgggcagcg cgctggtgca gaggatgaag
     1021 tgctacgcct cggaccagga ggtgctggac atgcacggtt tcctgcgcca ggcgctctgc
     1081 cgcctgcgcc aggaggagcc ccagagcctg caagctgccg tgcgcaccga tggcttcgac
     1141 gagttcaagg tgcgcctgca ggacctcagc tcttgcatca cccaggggaa agatgcagct
     1201 gtatccaaga aagccagccc agaggctgcc agcactccca gggaccctat tgacgttgac
     1261 ctgcccgagg aggcagagag agtgaaggcc caggttcagg ccctggggct ggctgaagcc
     1321 cagcctatgg ctgtggtaca gtcagtgccc ggggcacacc ccgtgccagt gtacgccttc
     1381 tccatcaaag gcccttccta tggagaggat gtctccaata caacgacagc ccagaagagg
     1441 aagtgcagcc agacccagtg ccccaggaag gtcatcaaga tggagtctga ggaggggaag
     1501 gaggcaaggt tggctcggag ctccccggag cagcccaggc ccagcacctc caaggcagtc
     1561 tcaccacccc acctggatgg accgcctagc cccaggagcc ccgtcatagg aagtgaggtc
     1621 ttcctgccca acagcaacca cgtggccagt ggcgccgggg aggcagagga acgcgttgtg
     1681 gtgatcagca gctcggaaga ctcagatgcc gaaaactcgt cctcccgaga gctggatgac
     1741 agcagcagtg agtccagtga cctccagctg gaaggcccca gcaccctcag ggtcctggac
     1801 gagaaccttg ctgaccccca agcagaagac agacctctgg ttttctttga cctcaagatt
     1861 gacaatgaaa cccagaagat tagccagctg gctgcggtga accgggaaag caagttccgc
     1921 gtggtcatcc agcctgaagc cttcttcagc atctactcca aggccgtgtc cctggaggtg
     1981 gggctgcagc acttcctcag ctttctgagc tccatgcgcc gccctatctt ggcctgctac
     2041 aagctgtggg ggcctggcct cccaaacttc ttccgggccc tggaggacat taacaggctg
     2101 tgggaattcc aggaggccat ctcgggcttc ctggctgccc tgcctctcat ccgggagcgt
     2161 gtgcccgggg ccagcagctt caaactcaag aacctggccc agacctacct ggcgagaaac
     2221 atgagcgagc gcagcgccat ggctgccgtg ctggccatgc gtgacctgtg ccgcctcctc
     2281 gaggtctccc cgggccccca gctggcccag catgtctacc ccttcagtag cctgcagtgc
     2341 tttgcctccc tgcagcccct ggtgcaggca gctgtgctgc cccgggctga ggcccgcctc
     2401 ctggccctac acaacgtgag cttcatggag ctgctgagtg cacaccgccg tgaccggcag
     2461 gggggcctga agaagtacag ccgctatcta agcctgcaga ccaccacgtt gccccctgcc
     2521 cagcctgctt tcaacctgca ggctctgggc acctactttg aaggcctgtt ggagggtccg
     2581 gcgctggcac gggcagaagg agtctccacc ccacttgctg gccgtggctt ggcagagagg
     2641 gcctcccagc agagctagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2701 ctttatatat cttgtggaaa ggacgacctg gagataggtt aagcacgtta
//
