LOCUS       sequence-279461-        1397 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0039
FEATURES             Location/Qualifiers
     source          1..1397
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtcctc cctgcttgag gaggaagttc actatggctc cagtcccctg
       61 gccatgctga cggcagcgtg cagcaaattt ggtggctcta gccctctgcg ggactcaaca
      121 actctgggca aagcaggcac aaagaagccg tactctgtgg gcagtgacct ttcagcctcc
      181 aaaaccatgg gggatgctta tccagccccc tttacaagca ctaatgggct cctttcacct
      241 gcaggcagtc ctccagcacc cacctcaggc tatgctaatg attaccctcc cttttcccac
      301 tcattccctg ggcccacagg cacccaggac cctgggctac tagtgcccaa ggggcacagc
      361 tcttctgact gtctgcccag tgtctacacc tctctggaca tgacacaccc ctatggctcc
      421 tggtacaagg caggcatcca tgcaggcatt tcaccaggcc caggcaacac tcctactcca
      481 tggtgggata tgcaccctgg aggcaactgg ctaggtggtg ggcagggcca gggtgatggg
      541 ctgcaaggga cactgcccac aggtccagct cagcctccac tgaaccccca gctgcccacc
      601 tacccatctg actttgctcc ccttaatcca gccccctacc cagctcccca cctcttgcaa
      661 ccagggcccc agcatgtctt gccccaagat gtctataaac ccaaggcagt gggaaatagt
      721 gggcagctag aagggagtgg tggagccaaa cccccacggg gtgcaagcac tgggggtagt
      781 ggtggatatg ggggcagtgg ggcagggcgc tcctcctgcg actgccctaa ttgccaggag
      841 ctagagcggc tgggagcagc agcggctggg ctgcggaaga agcccatcca cagctgccac
      901 atccctggct gcggcaaggt gtatggcaag gcttcgcacc tgaaggccca cttgcgctgg
      961 cacacaggcg agaggccctt cgtctgcaac tggctcttct gcggcaagag gttcactcgt
     1021 tcggatgagc tggagcgtca tgtgcgcact cacacccggg agaagaagtt cacctgcctg
     1081 ctctgctcca agcgctttac ccgaagcgac cacctgagca aacaccagcg cacccatgga
     1141 gaaccaggcc cgggtccccc tcccagtggc cccaaggagc tgggggaggg ccgcagcacg
     1201 ggggaagagg aggccagtca gacgccccga ccttctgcct cgccagcaac cccagagaaa
     1261 gcccctggag gcagccctga gcagagcaac ttgctggaga tctagtaatg aactagtccg
     1321 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgatacggca
     1381 gaggagtact cggtccg
//
