LOCUS       sequence-279470-        3287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0068
FEATURES             Location/Qualifiers
     source          1..3287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagaccc ggggtcggag ataatagaat ctgtccctcc agctggccct
       61 gaggcatctg agtcaacaac ggatgaaaat gaagacgaca ttcagtttgt cagtgaagga
      121 ccattacgac ctgttcttga atacattgat ctggtcagca gtgatgatga agagcctagc
      181 acctcttata ctgatgagaa tattaaacgt aaagaccata ttgattatca gaaggataaa
      241 gttgctttaa ctctggctcg tctagcccgc catgttgaag tggagaaaca gcagaaagaa
      301 gagaagaata gagcattcag agaaaaaatt gattttcagc atgctcatgg gttacaagaa
      361 ttggaattta ttcgaggaca ttctgataca gaagcagcaa gactgtgtgt ggaccagtgg
      421 ctaaaaatgc caggactcaa aacaggcaca attaattgtg gaacaaaaag ttcattccga
      481 agaggaggcc acacgtgggt gtctgggaaa ccaattttat gtcctataat gcactgtaac
      541 aaggagtttg acaatgggca ccttctctta ggacatttga aaaggttcga tcactctcca
      601 tgtgatccaa caattacact acatggacct ttcttcagct cctttgcttg tgtagtatgt
      661 tataaaaaat ttgttactca acaacaatat agagatcacc tttttgataa ggaagccaca
      721 gatgatggac ataacaacaa ccttcttcct cagattattc agtgttttgc atgtccaaat
      781 tgcttccttc tttttagcag aaaggaggag tgttcaaagc atatgtctgg aaagaatcat
      841 ttccatcaga gtttcaaact gggtgataac aaaggaattg cacatccaat atctttccca
      901 tcttttgcaa agaaactttt gatctctctg tgcaaagatg ttccctttca agttaagtgt
      961 gtggcctgcc acaagacact gcgttcccac atggagctca ctgcccattt cagagttcat
     1021 tgtcgaaatg ctggacctgt agctgtagct gagaagagca ttacccaggt tgcagagaaa
     1081 ttcatattaa gaggttattg tccagattgc aatcaagtct ttgtggatga aaccagcacc
     1141 caaaatcata agcagaattc aggacacaaa gtccgagtca ttaactcagt ggaagaatca
     1201 gtcttactct attgccacag cagcgaaggg aacaaggatc cttcttctga cttgcattta
     1261 ttgttggatc aatcaaaatt ttcatcactt aaaagaacca tgtctattaa agaatctagc
     1321 tcactggagt gcattgccat tccaaaaaag aagatgaatt taaaagataa aagccatgaa
     1381 ggtgttgctt gtgtccagaa agaaaaatca gtagttaaaa cctggttctg tgaatgcaat
     1441 cagcgattcc caagtgaaga tgcagtagaa aagcatgttt tctcagcaaa cacaatgggt
     1501 tataaatgtg tggtctgtgg aaaggtatgt gatgattcag gggtcattcg tttacacatg
     1561 agccggattc acggaggggc acatttaaat aactttcttt tctggtgtcg gacatgcaaa
     1621 aaggagttaa caaggaaaga tactatcatg gcacatgtga ctgaatttca taatggacac
     1681 agatattttt atgagatgga tgaggtagaa ggtgaaactt tgccatcatc ctctacaaca
     1741 ttggataatt tgactgctaa caagccttca tcagctatta ctgttattga tcattccccg
     1801 gcaaatagtt ctccgagggg taaatggcaa tgccggattt gtgaagatat gtttgattcc
     1861 caggaatatg taaaacagca ctgcatgtct ttggcaagcc acaagtttca tagatacagc
     1921 tgtgctcact gcagaaagcc ttttcataag atagaaacat tgtaccgaca ttgccaagat
     1981 gagcatgaca atgagataaa gattaaatac ttctgtgggc tttgtgatct tatctttaat
     2041 gtggaagaag catttctgag tcattatgag gagcaccaca gcatagatta tgtatttgtg
     2101 tcagaaaaaa ctgaaacttc aattaaaacc gaagatgatt ttccagtaat agagaccagt
     2161 aaccagttaa cttgtggttg ccgtgagagt tacatctgta aagtcaacag aaaagaagat
     2221 tatagcagat gtctccaaat catgctggat aaaggaaaac tgtggtttcg ctgcagttta
     2281 tgttcggcaa cagcacagaa tttaaccgac atgaacactc atatccatca agtgcacaaa
     2341 gaaaagagtg atgaggagga gcagcagtat gtaatcaagt gtggcacctg caccaaagca
     2401 tttcatgatc ctgagagtgc acagcagcat ttccatagaa aacattgctt cttacagaaa
     2461 cccagtgtgg ctcattttgg atctgaaaaa tcaaacctgt acaagtttac tgctagtgcc
     2521 tcacatacag agagaaaact gaaacaggca ataaactatt caaaaagttt agacatggag
     2581 aaaggagttg agaatgacct aagctatcag aatatagagg aagaaattgt tgagcttcca
     2641 gatttggatt acctgcgaac catgactcat atagtctttg tagattttga taactggtca
     2701 aacttttttg gtcatctacc agggcatcta aaccaaggaa catttatttg gggctttcaa
     2761 ggaggaaaca ccaattggaa gcctccgctc aactgtaaga tttataacta cctgaacagg
     2821 attggatgct tcttccttca tcctcgctgt agtaaaagaa aagatgctgc tgattttgcc
     2881 atatgtatgc atgctggccg tctagatgaa caactaccca agcaaattcc tttcaccatc
     2941 ctctcaggag atcaaggttt tctggagcta gagaatcaat ttaagaagac tcagaggcca
     3001 gctcatatac taaaccctca ccacttagag ggagatatga tgtgtgcctt gttaaatagc
     3061 atatctgata ccaccaaaga atgtgacagt gatgataaca tgggtgccaa aaatacttca
     3121 ataggagaag aatttatatc cacagaagat gtggaattag aagaagctat tagaagaagt
     3181 cttgaggaaa tgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     3241 tatatatctt gtggaaagga cgatcgcagt gggaaagacc tcgatat
//
