LOCUS       sequence-279476-        2543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0071
FEATURES             Location/Qualifiers
     source          1..2543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagaacgt ttttactgtg tcatcagatc ctcatccctc tcctgcagcc
       61 cctccttcac tttctttacc cttatcttca tcttctacct catctgggac taaaaaacaa
      121 aagaggaccc caacttatca gagatctatg agctttgatc caaaccttct ccacaacaat
      181 ggacataatg ggtaccctaa tggtacttca gcagcactgc gtgaaactgg ggttattgaa
      241 aaactgttaa cctcttacgg atttattcag tgttcagaac gtcaagctag acttttcttc
      301 cactgttcac agtataatgg caacctgcaa gacttaaaag taggagatga tgttgaattt
      361 gaagtatcat cggaccgacg gactgggaaa cccattgctg ttaaactggt gaagataaaa
      421 caagaaatcc tccctgaaga acgaatgaat ggacaagaag tgttttatct gacttacacc
      481 cctgaagatg tcgaagggaa cgttcagctg gaaactggag ataaaataaa ctttgtaatt
      541 gataacaata aacatactgg tgctgtaagt gctcgcaaca ttatgctgtt gaaaaagaaa
      601 caagcccgct gtcagggagt agtttgtgcc atgaaggagg catttggctt tattgaaaga
      661 ggtgatgttg taaaagagat attctttcac tatagtgaat ttaagggtga cttagaaacc
      721 ttacagcctg gcgatgatgt ggaattcaca atcaaggaca gaaatggtaa agaagttgca
      781 acagatgtca gactattgcc tcaaggaaca gtcatttttg aagatatcag cattgaacat
      841 tttgaaggaa ctgtaaccaa agttatccca aaagtaccca gtaaaaacca gaatgaccca
      901 ttgccaggac gcatcaaagt tgactttgtg atccctaaag aacttccctt tggagacaaa
      961 gatacgaaat ccaaggtgac cctgctggaa ggtgaccatg ttaggtttaa tatttcaaca
     1021 gaccgacgtg acaaattaga gcgagcaacc aatatagaag ttctgtcaaa tacatttcag
     1081 ttcactaatg aagcccgaga aatgggtgtg attgctgcca tgagagatgg ttttggtttc
     1141 atcaagtgtg tggatcgtga tgttcgtatg ttcttccact tcagtgaaat tctggatggg
     1201 aaccagctcc atattgcaga tgaagtagag tttactgtgg ttcctgatat gctctctgct
     1261 caaagaaatc atgctattag gattaaaaaa cttcccaagg gcacggtttc atttcattcc
     1321 cattcagatc accgttttct gggcacggta gaaaaagaag ccactttttc caatcctaaa
     1381 accactagcc caaataaagg caaagagaag gaggctgagg atggcattat tgcttatgat
     1441 gactgtgggg tgaaactgac tattgctttt caagccaagg atgtggaagg atctacttct
     1501 cctcaaatag gagataaggt tgaatttagt attagtgaca aacagaggcc tggacagcag
     1561 gttgcaactt gtgtgcgact tttaggtcgt aattctaact ccaagaggct cttgggttat
     1621 gtggcaactc tgaaggataa ttttggattt attgaaacag ccaatcatga taaggaaatc
     1681 tttttccatt acagtgagtt ctctggtgat gttgatagcc tggaactggg ggacatggtc
     1741 gagtatagct tgtccaaagg caaaggcaac aaagtcagtg cagaaaaagt gaacaaaaca
     1801 cactcagtga atggcattac tgaggaagct gatcccacca tttactctgg caaagtaatt
     1861 cgccccctga ggagtgttga tccaacacag actgagtacc aaggaatgat tgagattgtg
     1921 gaggagggcg atatgaaagg tgaggtctat ccatttggca tcgttgggat ggccaacaaa
     1981 ggggattgcc tgcagaaagg ggagagcgtc aagttccaat tgtgtgtcct gggccaaaat
     2041 gcacaaacta tggcttacaa catcacaccc ctgcgcaggg ccacagtgga atgtgtgaaa
     2101 gatcagtttg gcttcattaa ctatgaagta ggagatagca agaagctctt tttccatgtg
     2161 aaagaagttc aggatggcat tgagctacag gcaggagatg aggtggagtt ctcagtgatt
     2221 cttaatcagc gcactggcaa gtgcagcgcc tgtaatgttt ggcgagtctg tgagggcccc
     2281 aaggctgttg cagctcctcg acctgatcgg ttggtcaatc gcttgaagaa tatcactctg
     2341 gatgatgcca gtgctcctcg cctaatggtt cttcgtcagc caaggggacc agataactca
     2401 atggggtttg gtgcagaaag aaagatccgt caagctggtg tcattgacta gtaatgaact
     2461 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     2521 aatacacaaa ccgatttatt gac
//
