LOCUS       sequence-279491-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0080
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcatcca gggcatggag ctgtgcgcca tggccgtggt ggtgctgctg
       61 ttcatcgccg tcctcaagca gttcggcatc ctggagccca tatccatgga agatagcagc
      121 gacagtgagc tggagctgtc cacggtgcgc caccagccag aggggctgga ccagctgcag
      181 gcccagacca agttcaccaa gaaggagctg cagtctctct acaggggctt taagaatgag
      241 tgtcccacgg gcctggtgga cgaagacacc ttcaaactca tttacgcgca gttcttccct
      301 cagggagatg ccaccaccta tgcacacttc ctcttcaacg cctttgatgc ggacgggaac
      361 ggggccatcc actttgagga ctttgtggtt ggcctctcca tcctgctgcg gggcacagtc
      421 cacgagaagc tcaagtgggc ctttaatctc tacgacatta acaaggatgg ctacatcacc
      481 aaagaggaga tgctggccat catgaagtcc atctatgaca tgatgggccg ccacacctac
      541 cccatcctgc gggaggacgc gccggcggag cacgtggaga ggttcttcga gaaaatggac
      601 cggaaccagg atggggtagt gaccattgaa gagttcctgg aggcctgtca gaaggatgag
      661 aacatcatga gctccatgca gctgtttgag aatgtcatct agtaatgaac tagtccgtaa
      721 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tcgtatagtc
      781 tttggccgtc atca
//
