LOCUS       sequence-279506-        3416 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0090
FEATURES             Location/Qualifiers
     source          1..3416
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggatgg ccccaggtgt aagcgcagaa agcaggcgaa cccgcggcgc
       61 aataacgtta caaattataa tactgtggta gaaacaaatt cagattcaga tgatgaagac
      121 aaactgcata ttgtggaaga agaaagtgtt acagatgcag ctgactgtga aggtgtacca
      181 gaggatgacc tgccaacaga ccagacagtg ttaccaggga ggagcagtga aagagaaggg
      241 aatgctaaga actgctggga ggatgacata aaagatgatg aatgcgagtc agatgcagaa
      301 aatgagcaaa accatgatcc taatgttgaa gagtttctac aacaacaaga cactgctgtc
      361 atttttcctg aggcacctga agaggaccag aggcagggca caccagaagc cagtggtcat
      421 gatgaaaatg gaacaccaga tgcattttca caattactca cctgtccata ttgtgataga
      481 ggctataaac gctttacctc tctgaaagaa cacattaaat atcgtcatga aaagaatgaa
      541 gataacttta gttgctccct gtgcagttac acctttgcat acagaaccca acttgaacgt
      601 cacatgacat cacataaatc aggaagagat caaagacatg tgacgcagtc tgggtgtaat
      661 cgtaaattca aatgcactga gtgtggaaaa gctttcaaat acaaacatca cctaaaagag
      721 cacttaagaa ttcacagtgg agagaagcca tatgaatgcc caaactgcaa gaaacgcttt
      781 tcccattctg gctcctatag ctcacacata agcagtaaga aatgtatcag cttgatacct
      841 gtgaatgggc gaccaagaac aggactcaag acatctcagt gttcttcacc gtctctttca
      901 gcatcaccag gcagtcccac acgaccacag atacggcaaa agatagagaa taaacccctt
      961 caagaacaac tttctgttaa ccaaattaaa actgaacctg tggattatga attcaaaccc
     1021 atagtggttg cttcaggaat caactgttca acccctttac aaaatggggt tttcactggt
     1081 ggtggcccat tacaggcaac cagttctcct cagggcatgg tgcaagctgt tgttctgcca
     1141 acagttggtt tggtgtctcc cataagtatc aatttaagtg atattcagaa tgtacttaaa
     1201 gtggcggtag atggtaatgt aataaggcaa gtgttggaga ataatcaagc caatcttgca
     1261 tccaaagaac aagaaacaat caatgcttca cccatacaac aaggtggcca ttctgttatt
     1321 tcagccatca gtcttccttt ggttgatcaa gatggaacaa ccaaaattat catcaactac
     1381 agtcttgagc agcctagcca acttcaagtt gttcctcaaa atttaaaaaa agaaaatcca
     1441 gtcgctacaa acagttgtaa aagtgaaaag ttaccagaag atcttactgt taagtctgag
     1501 aaggacaaaa gctttgaagg gggggtgaat gatagcactt gtcttctgtg tgatgattgt
     1561 ccaggagata ttaatgcact tccagaatta aagcactatg acctaaagca gcctactcag
     1621 cctcctccac tccctgcagc agaagctgag aagcctgagt cctctgtttc atcagctact
     1681 ggagatggca atttgtctcc tagtcagcca cctttaaaga acctcttgtc tctcctaaaa
     1741 gcatattatg ctttgaatgc acaaccaagt gcagaagagc tctcaaaaat tgctgattca
     1801 gtaaacctac cactggatgt agtaaaaaag tggtttgaaa agatgcaagc tggacagatt
     1861 tcagtgcagt cttctgaacc atcttctcct gaaccaggca aagtaaatat ccctgccaag
     1921 aacaatgatc agcctcaatc tgcaaatgca aatgaacccc aggacagcac agtaaatcta
     1981 caaagtcctt tgaagatgac taactcccca gttttaccag tgggatcaac caccaatggt
     2041 tccagaagta gtacaccatc cccatcacct ctaaaccttt cctcatccag aaatacacag
     2101 ggttacttgt acacagctga gggtgcacaa gaagagccac aagtagaacc tcttgatctt
     2161 tcactaccaa agcaacaggg agaattatta gaaaggtcaa ctatcactag tgtttaccag
     2221 aacagtgttt attctgtcca ggaagaaccc ttgaacttgt cttgcgcaaa aaaggagcca
     2281 caaaaggaca gttgtgttac agactcagaa ccagttgtaa atgtaatccc accaagtgcc
     2341 aaccccataa atatcgctat acctacagtc actgcccagt tacccacaat cgtggccatt
     2401 gctgaccaga acagtgttcc atgcttaaga gcgctagctg ccaataagca aacgattctg
     2461 attccccagg tggcatacac ctactcaact acggtcagcc ctgcagtcca agaaccaccc
     2521 ttgaaagtga tccagccaaa tggaaatcag gatgaaagac aagatactag ctcagaagga
     2581 gtatcaaatg tagaggatca gaatgactct gattctacac cgcccaaaaa gaaaatgcgg
     2641 aagacagaaa atggaatgta tgcttgtgat ttgtgtgaca agatattcca aaagagtagt
     2701 tcattattga gacataaata tgaacacaca ggtaaaagac ctcatgagtg tggaatctgt
     2761 aaaaaggcat ttaaacacaa acatcatttg attgaacaca tgcgattaca ttctggagaa
     2821 aagccctatc aatgtgacaa atgtggaaag cgcttctcac actctgggtc ttattctcaa
     2881 cacatgaatc atcgctactc ctactgtaag agagaagcgg aagaacgtga cagcacagag
     2941 caggaagagg cagggcctga aatcctctcg aatgagcacg tgggtgccag ggcgtctccc
     3001 tcacagggcg actcggacga gagagagagt ttgacaaggg aagaggatga agacagtgaa
     3061 aaagaggaag aggaggagga taaagagatg gaagaattgc aggaagaaaa agaatgtgaa
     3121 aaaccacaag gggatgagga agaggaggag gaggaggaag aagtggaaga agaagaggta
     3181 gaagaggcag agaatgaggg agaagaagca aaaactgaag gtctgatgaa ggatgacagg
     3241 gctgaaagtc aagcaagcag cttaggacaa aaagtaggcg agagtagtga gcaagtgtct
     3301 gaagaaaaga caaatgaagc ctagtaatga actagtccgt aacttgaaag tatttcgatt
     3361 tcttggcttt atatatcttg tggaaaggac gactaactcc tgttatggcg ctaggc
//
