LOCUS       sequence-279509-        3479 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0091
FEATURES             Location/Qualifiers
     source          1..3479
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggatgg ccccaggtgt aagcgcagaa agcaggcgaa cccgcggcgc
       61 aataacgtta caaattataa tactgtggta gaaacaaatt cagattcaga tgatgaagac
      121 aaactgcata ttgtggaaga agaaagtgtt acagatgcag ctgactgtga aggtgtacca
      181 gaggatgacc tgccaacaga ccagacagtg ttaccaggga ggagcagtga aagagaaggg
      241 aatgctaaga actgctggga ggatgacaca ggaaaggaag ggcaagaaat cctggggcct
      301 gaagctcagg cagatgaagc aggatgtaca gtaaaagatg atgaatgcga gtcagatgca
      361 gaaaatgagc aaaaccatga tcctaatgtt gaagagtttc tacaacaaca agacactgct
      421 gtcatttttc ctgaggcacc tgaagaggac cagaggcagg gcacaccaga agccagtggt
      481 catgatgaaa atggaacacc agatgcattt tcacaattac tcacctgtcc atattgtgat
      541 agaggctata aacgctttac ctctctgaaa gaacacatta aatatcgtca tgaaaagaat
      601 gaagataact ttagttgctc cctgtgcagt tacacctttg catacagaac ccaacttgaa
      661 cgtcacatga catcacataa atcaggaaga gatcaaagac atgtgacgca gtctgggtgt
      721 aatcgtaaat tcaaatgcac tgagtgtgga aaagctttca aatacaaaca tcacctaaaa
      781 gagcacttaa gaattcacag tggagagaag ccatatgaat gcccaaactg caagaaacgc
      841 ttttcccatt ctggctccta tagctcacac ataagcagta agaaatgtat cagcttgata
      901 cctgtgaatg ggcgaccaag aacaggactc aagacatctc agtgttcttc accgtctctt
      961 tcagcatcac caggcagtcc cacacgacca cagatacggc aaaagataga gaataaaccc
     1021 cttcaagaac aactttctgt taaccaaatt aaaactgaac ctgtggatta tgaattcaaa
     1081 cccatagtgg ttgcttcagg aatcaactgt tcaacccctt tacaaaatgg ggttttcact
     1141 ggtggtggcc cattacaggc aaccagttct cctcagggca tggtgcaagc tgttgttctg
     1201 ccaacagttg gtttggtgtc tcccataagt atcaatttaa gtgatattca gaatgtactt
     1261 aaagtggcgg tagatggtaa tgtaataagg caagtgttgg agaataatca agccaatctt
     1321 gcatccaaag aacaagaaac aatcaatgct tcacccatac aacaaggtgg ccattctgtt
     1381 atttcagcca tcagtcttcc tttggttgat caagatggaa caaccaaaat tatcatcaac
     1441 tacagtcttg agcagcctag ccaacttcaa gttgttcctc aaaatttaaa aaaagaaaat
     1501 ccagtcgcta caaacagttg taaaagtgaa aagttaccag aagatcttac tgttaagtct
     1561 gagaaggaca aaagctttga agggggggtg aatgatagca cttgtcttct gtgtgatgat
     1621 tgtccaggag atattaatgc acttccagaa ttaaagcact atgacctaaa gcagcctact
     1681 cagcctcctc cactccctgc agcagaagct gagaagcctg agtcctctgt ttcatcagct
     1741 actggagatg gcaatttgtc tcctagtcag ccacctttaa agaacctctt gtctctccta
     1801 aaagcatatt atgctttgaa tgcacaacca agtgcagaag agctctcaaa aattgctgat
     1861 tcagtaaacc taccactgga tgtagtaaaa aagtggtttg aaaagatgca agctggacag
     1921 atttcagtgc agtcttctga accatcttct cctgaaccag gcaaagtaaa tatccctgcc
     1981 aagaacaatg atcagcctca atctgcaaat gcaaatgaac cccaggacag cacagtaaat
     2041 ctacaaagtc ctttgaagat gactaactcc ccagttttac cagtgggatc aaccaccaat
     2101 ggttccagaa gtagtacacc atccccatca cctctaaacc tttcctcatc cagaaataca
     2161 cagggttact tgtacacagc tgagggtgca caagaagagc cacaagtaga acctcttgat
     2221 ctttcactac caaagcaaca gggagaatta ttagaaaggt caactatcac tagtgtttac
     2281 cagaacagtg tttattctgt ccaggaagaa cccttgaact tgtcttgcgc aaaaaaggag
     2341 ccacaaaagg acagttgtgt tacagactca gaaccagttg taaatgtaat cccaccaagt
     2401 gccaacccca taaatatcgc tatacctaca gtcactgccc agttacccac aatcgtggcc
     2461 attgctgacc agaacagtgt tccatgctta agagcgctag ctgccaataa gcaaacgatt
     2521 ctgattcccc aggtggcata cacctactca actacggtca gccctgcagt ccaagaacca
     2581 cccttgaaag tgatccagcc aaatggaaat caggatgaaa gacaagatac tagctcagaa
     2641 ggagtatcaa atgtagagga tcagaatgac tctgattcta caccgcccaa aaagaaaatg
     2701 cggaagacag aaaatggaat gtatgcttgt gatttgtgtg acaagatatt ccaaaagagt
     2761 agttcattat tgagacataa atatgaacac acaggtaaaa gacctcatga gtgtggaatc
     2821 tgtaaaaagg catttaaaca caaacatcat ttgattgaac acatgcgatt acattctgga
     2881 gaaaagccct atcaatgtga caaatgtgga aagcgcttct cacactctgg gtcttattct
     2941 caacacatga atcatcgcta ctcctactgt aagagagaag cggaagaacg tgacagcaca
     3001 gagcaggaag aggcagggcc tgaaatcctc tcgaatgagc acgtgggtgc cagggcgtct
     3061 ccctcacagg gcgactcgga cgagagagag agtttgacaa gggaagagga tgaagacagt
     3121 gaaaaagagg aagaggagga ggataaagag atggaagaat tgcaggaaga aaaagaatgt
     3181 gaaaaaccac aaggggatga ggaagaggag gaggaggagg aagaagtgga agaagaagag
     3241 gtagaagagg cagagaatga gggagaagaa gcaaaaactg aaggtctgat gaaggatgac
     3301 agggctgaaa gtcaagcaag cagcttagga caaaaagtag gcgagagtag tgagcaagtg
     3361 tctgaagaaa agacaaatga agcctagtaa tgaactagtc cgtaacttga aagtatttcg
     3421 atttcttggc tttatatatc ttgtggaaag gacgaagaga gttggccctc gagtaagag
//
