LOCUS       sequence-27951-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Battesti A, Bouveret E
  TITLE     Improvement of bacterial two-hybrid vectors for detection of fusion 
            proteins and transfer to pBAD-tandem affinity purification, 
            calmodulin binding peptide, or 6-histidine tag vectors.
  JOURNAL   Proteomics. 2008 Nov . 8(22):4768-71.
  PUBMED    18924111
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteomics.
            2008 Nov . 8(22):4768-71."
COMMENT     Sequence Label: pEB587
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             102..275
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             276..449
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             486..506
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             513..590
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     primer_bind     complement(670..687)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagcagg
       61 aggaattcac catggcaggc cttgcgcaac acgatgaagc cgtggacaac aaattcaaca
      121 aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac
      181 gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag
      241 aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga caacaaattc aacaaagaac
      301 aacaaaacgc gttctatgag atcttacatt tacctaactt aaacgaagaa caacgaaacg
      361 ccttcatcca aagtttaaaa gatgacccaa gccaaagcgc taacctttta gcagaagcta
      421 aaaagctaaa tgatgctcag gcgccgaaag tagacgcgaa ttgtgatata cctacaactg
      481 cttctgaaaa tttatatttt caaggtgaac taaagagaag atggaaaaag aatttcatag
      541 ccgtctcagc agccaaccgc tttaagaaaa tctcatcctc cggggcactt gtcgagtcga
      601 ctctagactc gagttcgacc tgcaggcatg caagcttggc tgttttggcg gatgagagaa
      661 gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
      721 gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
      781 ccgta
//
