LOCUS       sequence-27952-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Battesti A, Bouveret E
  TITLE     Improvement of bacterial two-hybrid vectors for detection of fusion 
            proteins and transfer to pBAD-tandem affinity purification, 
            calmodulin binding peptide, or 6-histidine tag vectors.
  JOURNAL   Proteomics. 2008 Nov . 8(22):4768-71.
  PUBMED    18924111
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteomics.
            2008 Nov . 8(22):4768-71."
COMMENT     Sequence Label: pEB1029
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             669..692
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgcatcaggc tggttacgca aacgccgccg accgggagtc tggcatcccc gcagccgtac
       61 tcgatggcat caaggccgtg gcgaaggaaa aaaacgccac attgatgttc cgcctggtca
      121 acccccattc caccagcctg attgccgaag gggtggccac caaaggattg ggcgtgcacg
      181 ccaagtcgtc cgattggggg ttgcaggcgg gctacattcc cgtcaacccg aatctttcca
      241 aactgttcgg ccgtgcgccc gaggtgatcg cgcgggccga caacgacgtc aacagcagcc
      301 tggcgcatgg ccataccgcg gtcgacctga cgctgtcgaa agagcggctt gactatctgc
      361 ggcaagcggg cctggtcacc ggcatggccg atggcgtggt cgcgagcaac cacgcaggct
      421 acgagcagtt cgagtttcgc gtgaaggaaa cctcggacgg gcgctatgcc gtgcagtatc
      481 gccgcaaggg cggcgacgat ttcgaggcgg tcaaggtgat cggcaatgcc gccggtattc
      541 cactgagggc ggatatcgac atgttcgcca ttatgccgca tctgtccaac ttccgcgact
      601 cggcgcgcag ttcggtgacc agcggcgatt cggtgaccga ttacctggcg cgcacgcggc
      661 gggctgcaga ttataaagat gacgatgaca agctagaatt cgatatcaag cttatcgata
      721 ccgtcga
//
