LOCUS       sequence-27953-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Battesti A, Bouveret E
  TITLE     Improvement of bacterial two-hybrid vectors for detection of fusion 
            proteins and transfer to pBAD-tandem affinity purification, 
            calmodulin binding peptide, or 6-histidine tag vectors.
  JOURNAL   Proteomics. 2008 Nov . 8(22):4768-71.
  PUBMED    18924111
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteomics.
            2008 Nov . 8(22):4768-71."
COMMENT     Sequence Label: pEB1030
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             519..542
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(566..582)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(567..583)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(625..644)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgggcggcct ggatcgcgaa cgcatcgact tgttgtggaa aatcgctcgc gccggcgccc
       61 gttccgcagt gggcaccgag gcgcgtcgcc agttccgcta cgacggcgac atgaatatcg
      121 gcgtgatcac cgatttcgag ctggaagtgc gcaatgcgct gaacaggcgg gcgcacgccg
      181 tcggcgcgca ggacgtggtc cagcatggca ctgagcagaa caatcctttc ccggaggcag
      241 atgagaagat tttcgtcgta tcggccaccg gtgaaagcca gatgctcacg cgcgggcaac
      301 tgaaggaata cattggccag cagcgcggcg agggctatgt cttctacgag aaccgtgcat
      361 acggcgtggc ggggaaaagc ctgttcgacg atgggctggg agccgcgccc ggcgtgccga
      421 gcggacgttc gaagttctcg ccggatgtac tggaaacggt gccggcgtca cccggattgc
      481 ggcggccgtc gctgggcgca gtggaacgcc actgcaggga ttataaagat gacgatgaca
      541 agctagaatt cgatatcaag cttatcgata ccgtcgacct cgagcaattc atcgatataa
      601 ctaagtaata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
      661 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
      721 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt ca
//
