LOCUS       sequence-279530-        1817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0147
FEATURES             Location/Qualifiers
     source          1..1817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgctcac cagcttttta cctgcaccta ctcagctatc tcaggaccag
       61 cttgaggctg aagaaaaggc aagatcccag agatcacggc agacctcact ggtctcctcc
      121 cgaagagaac ctcccccgta cggataccgg aaaggctgga tacctcggtt attagaggat
      181 tttggagatg gaggtgcttt tccagagatc catgtggccc agtatccact ggatatggga
      241 cgaaagaaaa aaatgtcgaa tgcgctggcc attcaggtgg attctgaagg aaaaattaaa
      301 tatgatgcaa ttgctcgaca aggacagtca aaagacaagg tcatttatag caaatacact
      361 gacctggttc caaaggaggt tatgaatgca gatgatccag acctgcaaag gcccgatgaa
      421 gaagctatta aagagataac agaaaagaca agagtagcct tagaaaaatc tgtatcacag
      481 aaggtcgccg cagccatgcc agttcgagca gctgacaaat tggctcctgc tcagtatatc
      541 cgatacacac catctcagca aggagtggca ttcaactctg gagctaaaca gagggttatt
      601 cggatggtag aaatgcagaa agatccaatg gagcctccaa ggttcaagat taataagaaa
      661 attccccggg gaccaccttc tcctcctgcg cctgtcatgc attctcctag ccgaaagatg
      721 actgtaaagg aacaacaaga gtggaagatt cctccttgta tttctaactg gaaaaatgca
      781 aagggttata caattccatt agacaaacgt ctggctgctg atggaagagg actacagaca
      841 gtacacataa atgaaaattt cgccaaattg gcagaagccc tctacattgc tgatcggaag
      901 gctcgtgaag ctgtggaaat gcgtgcccaa gtagagagaa aaatggctca gaaagaaaag
      961 gaaaaacatg aagagaaact tagagaaatg gcccagaaag ccagggagag aagagctggg
     1021 atcaaaactc atgtggaaaa agaggatggg gaggcacgtg agagggatga aatccggcat
     1081 gacaggcgaa aagagagaca gcatgaccgg aatctttcca gggcagctcc tgataagagg
     1141 tcgaaacttc agagaaatga aaatcgggat atcagtgaag ttattgctct cggtgttcct
     1201 aatcctcgga cttccaatga agttcagtat gaccaaaggc tcttcaacca atccaagggt
     1261 atggacagtg gatttgcagg tggagaagat gaaatttata atgtttatga tcaagcctgg
     1321 agaggtggta aagatatggc ccagagtatt tataggccca gtaaaaatct ggacaaggac
     1381 atgtatggtg atgacctaga agccagaata aagaccaaca ggtgccaagc catacaactc
     1441 aatttcagtg tttacactgg tgaaagcaaa gtagttcata gttttttctc cttttcctta
     1501 gatttgttcc cgacaaggag ttttctggtt cagaccgtag acagagaggc cgagaaggac
     1561 cagtgcagtt tgaggaagat ccttttggtt tggacaagtt tttggaagaa gccaaacagc
     1621 atggtggctc taaaagaccc tcagatagca gccgccccaa ggaacacgag catgaaggca
     1681 agaagaggag gaaggaatag gcacaggtct ctccaaagtg aatagtaatg aactagtccg
     1741 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgacgtccgt
     1801 cgtttcacct gtactac
//
