LOCUS       sequence-279545-        2072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0158
FEATURES             Location/Qualifiers
     source          1..2072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgtccac ggattacagt acctatagcc aagctgcagc gcagcagggc
       61 tacagtgctt acaccgccca gcccactcaa ggatatgcac agaccaccca ggcatatggg
      121 caacaaagct atggaaccta tggacagccc actgatgtca gctataccca ggctcagacc
      181 actgcaacct atgggcagac cgcctatgca acttcttatg gacagcctcc cactggttat
      241 actactccaa ctgcccccca ggcatacagc cagcctgtcc aggggtatgg cactggtgct
      301 tatgatacca ccactgctac agtcaccacc acccaggcct cctatgcagc tcagtctgca
      361 tatggcactc agcctgctta tccagcctat gggcagcagc cagcagccac tgcacctaca
      421 agaccgcagg atggaaacaa gcccactgag actagtcaac ctcaatctag cacagggggt
      481 tacaaccagc ccagcctagg atatggacag agtaactaca gttatcccca ggtacctggg
      541 agctacccca tgcagccagt cactgcacct ccatcctacc ctcctaccag ctattcctct
      601 acacagccga ctagttatga tcagagcagt tactctcagc agaacaccta tgggcaaccg
      661 agcagctatg gacagcagag tagctatggt caacaaagca gctatgggca gcagcctccc
      721 actagttacc caccccaaac tggatcctac agccaagctc caagtcaata tagccaacag
      781 agcagcagct acgggcagca gagttcattc cgacaggacc accccagtag catgggtgtt
      841 tatgggcagg agtctggagg attttccgga ccaggagaga accggagcat gagtggccct
      901 gataaccggg gcaggggaag agggggattt gatcgtggag gcatgagcag aggtgggcgg
      961 ggaggaggac gcggtggaat gggcagcgct ggagagcgag gtggcttcaa taagcctggt
     1021 ggacccatgg atgaaggacc agatcttgat ctaggcccac ctgtagatcc agatgaagac
     1081 tctgacaaca gtgcaattta tgtacaagga ttaaatgaca gtgtgactct agatgatctg
     1141 gcagacttct ttaagcagtg tggggttgtt aagatgaaca agagaactgg gcaacccatg
     1201 atccacatct acctggacaa ggaaacagga aagcccaaag gcgatgccac agtgtcctat
     1261 gaagacccac ccactgccaa ggctgccgtg gaatggtttg atgggaaaga ttttcaaggg
     1321 agcaaactta aagtctccct tgctcggaag aagcctccaa tgaacagtat gcggggtggt
     1381 ctgccacccc gtgagggcag aggcatgcca ccaccactcc gtggaggtcc aggaggccca
     1441 ggaggtcctg ggggacccat gggtcgcatg ggaggccgtg gaggagatag aggaggcttc
     1501 cctccaagag gaccccgggg ttcccgaggg aacccctctg gaggaggaaa cgtccagcac
     1561 cgagctggag actggcagtg tcccaatccg ggttgtggaa accagaactt cgcctggaga
     1621 acagagtgca accagtgtaa ggccccaaag cctgaaggct tcctcccgcc accctttccg
     1681 cccccgggtg gtgatcgtgg cagaggtggc cctggtggca tgcggggagg aagaggtggc
     1741 ctcatggatc gtggtggtcc cggtggaatg ttcagaggtg gccgtggtgg agacagaggt
     1801 ggcttccgtg gtggccgggg catggaccga ggtggctttg gtggaggaag acgaggtggc
     1861 cctggggggc cccctggacc tttgatggaa cagatgggag gaagaagagg aggacgtgga
     1921 ggacctggaa aaatggataa aggcgagcac cgtcaggagc gcagagatcg gccctactag
     1981 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2041 aaggacgaat actcgcgcgg ccgtcatcac ac
//
