LOCUS       sequence-279554-        1853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0163
FEATURES             Location/Qualifiers
     source          1..1853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaggcct tgagaagaag aagtatgaac gaggctcggc caccaactac
       61 atcacccgga acaaagcccg gaagaagctc cagctgagct tggctgactt taggcggctg
      121 tgcattctga agggcattta tccccatgaa cccaaacaca agaagaaggt taacaagggt
      181 tctacagcag cccgaacgtt ttaccttatc aaagacatca ggtttctcct ccacgaaccc
      241 attgtcaaca agttccgtga atacaaggtg ttcgtccgga agctccggaa ggcttatggg
      301 aagagcgagt ggaacactgt agagcgttta aaggacaata agcccaacta caaactcgac
      361 cacatcatca aggaacggta tcccacgttc atcgatgccc tgcgggacct ggacgatgcc
      421 ctctccatgt gcttcctgtt ttccaccttc ccgcggactg gcaagtgcca cgtgcagacc
      481 attcagctgt gccgccggct cactgtggag ttcatgcact acattatcgc tgcccgtgcc
      541 ctgcgcaagg tcttcctgtc catcaaaggc atttactacc aggccgaggt actggggcag
      601 cccatcgtgt ggatcactcc ctatgccttc tcccatgacc acccgacaga cgtggactac
      661 agggtcatgg ccaccttcac cgagttctac accacgctgc tgggctttgt caacttccgc
      721 ctttaccagt tgctcaacct ccactatccc ccgaagctcg agggtcaggc ccaagcagag
      781 gcaaaggccg gtgagggcac ctacgcgttg gactccgaga gttgtatgga gaaactggca
      841 gccctcagtg ccagcctggc ccgcgtggtg gtgcctgcca cagaggagga ggccgaggtg
      901 gatgagtttc ccaccgatgg ggaggaagac cgcaggaagg agctggaggc gcaggagaag
      961 cacaagaagc tttttgaggg cctgaagttc ttcctgaacc gagaggtgcc ccgtgaggcc
     1021 ctggccttca tcatcaggag ttttggtggg gaagtgtcct gggacaaatc tttgtgcatt
     1081 ggggccacct atgacgtcac agactcccgc atcacccatc agattgtcga ccggcctggg
     1141 cagcagacct cagtcattgg caggtgctac gtgcagcccc agtgggtgtt tgactcagtg
     1201 aacgccaggc tccttctccc cgtggcagag tacttctctg gggtgcagct gcccccacac
     1261 ctttcaccct ttgtgaccga gaaggaagga gattacgttc cacctgagaa gctgaagctg
     1321 ctggctctgc agcggggaga ggacccagga aacctgaatg agtcagaaga ggaggaggaa
     1381 gaggacgaca acaacgaagg tgatggtgat gaagagggag aaaatgagga ggaggaggaa
     1441 gatgcagagg ctggttcaga aaaggaggaa gaggcccggc tggcagccct ggaagagcag
     1501 aggatggagg ggaagaagcc cagggtgatg gcaggcacct tgaagctgga ggataagcag
     1561 cggctggccc aggaggagga gagtgaggcc aagcgcctgg ccattatgat gatgaagaag
     1621 cgggagaagt acctgtacca gaagatcatg tttggcaaga ggcgaaaaat ccgagaggcc
     1681 aacaagctgg cggagaagcg gaaagcccac gatgaggcgg tgaggtctga gaagaaggcc
     1741 aagaaggcaa ggccggagta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1801 tggctttata tatcttgtgg aaaggacgaa tcgcgtaaag tcgtccatct agt
//
