LOCUS       sequence-27956-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Gully D, Bouveret E
  TITLE     A protein network for phospholipid synthesis uncovered by a variant 
            of the tandem affinity purification method in Escherichia coli.
  JOURNAL   Proteomics. 2006 Jan . 6(1):282-93.
  PUBMED    16294310
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteomics.
            2006 Jan . 6(1):282-93."
COMMENT     Sequence Label: pEB602
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(47..124)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     promoter        complement(168..452)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(226..245)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 agcttgcatg cctgcaggtc gaactcgagt ctagagtcga ctcgacaagt gccccggagg
       61 atgagatttt cttaaagcgg ttggctgctg agacggctat gaaattcttt ttccatcttc
      121 tctttagttc catggtgaat tcctcctgct agcccaaaaa aacgggtatg gagaaacagt
      181 agagagttgc gataaaaagc gtcaggtagg atccgctaat cttatggata aaaatgctat
      241 ggcatagcaa agtgtgacgc cgtgcaaata atcaatgtgg acttttctgc cgtgattata
      301 gacacttttg ttacgcgttt ttgtcatggc tttggtcccg ctttgttaca gaatgctttt
      361 aataagcggg gttaccggtt tggttagcga gaagagccag taaaagacgc agtgacggca
      421 atgtctgatg caatatggac aattggtttc ttctctgaat ggcgggagta tgaaaagtat
      481 ggctgaagcg caaaatgatc ccctgctgcc gggatactcg tttaatgccc atctggtggc
      541 gggtttaacg ccgattgagg ccaacggtta tctcgatttt tttatcgacc gaccgctggg
      601 aatgaaaggt tatattctca atctcaccat tcgcggtcag ggggtggtga aaaatcaggg
      661 acgagaattt gtttgccgac cgggtgatat tttgctgttc ccgcc
//
