LOCUS       sequence-279566-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0183
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagaact tacggcgttc gtctccaagt cttttgacca gaaagtgaag
       61 gagaagaagg aggcgatcac gtaccgggag gtgctggaga gcgggccgct gcgcggggcc
      121 aaggagccga ccggctgcac cgaggcgggc cgcgacgacc gcagcagccc ggcagtccgg
      181 gcggccggcg gaggcggcgg cggaggaggc ggaggcggcg gcggaggagg cggaggaggt
      241 gtaggaggag gaggagcagg cggaggagct ggaggagggc gctctcccgt ccgggagctg
      301 gacatgggcg ccgccgagag aagcagggag ccgggcagcc cgcgactgac ggaggtgtcc
      361 ccggagctga aagatcgcaa agaggatgcg aaagggatgg aggacgaagg ccagaccaaa
      421 atcaagcaga ggcgaagtcg gaccaatttc accctggaac aactcaatga gctggagagg
      481 ctttttgacg agacccacta tcccgacgcc ttcatgcgag aggaactgag ccagcgactg
      541 ggcctgtcgg aggcccgagt gcaggtttgg tttcaaaatc gaagagctaa atgtagaaaa
      601 caagaaaatc aactccataa aggtgttctc ataggggccg ccagccagtt tgaagcttgt
      661 agagtcgcac cttatgtcaa cgtaggtgct ttaaggatgc catttcagca ggttcaggcg
      721 cagctgcagc tggacagcgc tgtggcgcac gcgcaccacc acctgcatcc gcacctggcc
      781 gcgcacgcgc cctacatgat gttcccagca ccgcccttcg gactgccgct cgccacgctg
      841 gccgcggatt cggcttccgc cgcctcggta gtggcggccg cagcagccgc caagaccacc
      901 agcaagaact ccagcatcgc cgatctcaga ctgaaagcca aaaagcacgc cgcagccctg
      961 ggtctgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1021 tcttgtggaa aggacgacac aaccctaacc cgaatgcgaa t
//
