LOCUS       sequence-279584-        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0205
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgcggcc tgtgagcgac aggaccccgg cccctctgct gctgggcggc
       61 ccggccggga caccccctgg cgggggagcg ctgcttgggt tgcggagcct tctgcagggg
      121 accagcaagc ccaaagagcc ggccagctgt ctcctgaagg aaaaggagcg caaggcggcc
      181 ctgcctgcag ccacaacccc tgggccaggc ctggagactg cgggcccggc ggatgccccg
      241 gctggggcag tggtgggcgg agggtccccg cgggggcgcc cggggccggt gcccgccccg
      301 ggtctgttgg cgccactgct gtgggagcgc acgctgccgt tcggcgatgt ggagtacgta
      361 gacctggacg ccttcctgct ggagcacggg ctcccgccca gcccgccgcc ccccggtggc
      421 ccgtcgccgg agccgtcgcc cgcgcggacg cccgcaccct ccccagggcc gggttcgtgc
      481 ggctcggctt ccccccgctc ctctcctggg cacgcccccg cccgggctgc cctcgggacc
      541 gccagcggcc accgcgcagg cctgacctct cgggacacac ccagccctgt ggacccagac
      601 accgtggagg tgttgatgac ctttgaaccc gacccagctg atcttgccct atcaagcatt
      661 cctggccacg agacctttga ccctcgaaga catcgcttct cagaagagga acttaagccc
      721 cagccaatca tgaagaaggc aagaaaaatc caggtgccgg aggagcagaa ggatgagaaa
      781 tactggagcc ggcggtacaa gaacaacgag gcagccaagc ggtcccgtga cgcccggcgg
      841 ctcaaggaga accagatatc ggtgcgggcg gccttcctgg agaaggagaa cgccctgctg
      901 cggcaggaag ttgtggccgt gcgccaggag ctgtcccact accgcgccgt gctgtcccga
      961 taccaggccc agcacggggc cctgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1021 atttcttggc tttatatatc ttgtggaaag gacgaactaa caatctccat ctcttaaca
//
