LOCUS       sequence-279593-        1835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0213
FEATURES             Location/Qualifiers
     source          1..1835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             508..516
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgatgacacc tcaagttatc actccccagc aaatgcagca gatcctccag
       61 caacaagtgc tgagccctca gcagctccag gttctcctcc agcagcagca ggccctcatg
      121 cttcaacagc agcagcttca agagttttat aaaaaacaac aggaacagtt gcagcttcaa
      181 cttttacaac aacaacatgc tggaaaacag cctaaagagc aacagcaggt ggctacccag
      241 cagttggctt ttcagcagca gcttttacag atgcagcagt tacagcagca gcacctcctg
      301 tctttgcagc gccaaggcct tctgacaatt cagcccgggc agcctgccct tccccttcaa
      361 cctcttgctc aaggcatgat tccaacagaa ctgcagcagc tctggaaaga agtgacaagt
      421 gctcatactg cagaagaaac cacaggcaac aatcacagca gtttggatct gaccacgaca
      481 tgtgtctcct cctctgcacc ttccaagacc tccttaataa tgaacccaca tgcctctacc
      541 aatggacagc tctcagtcca cactcccaaa agggaaagtt tgtcccatga ggagcacccc
      601 catagccatc ctctctatgg acatggtgta tgcaagtggc caggctgtga agcagtgtgc
      661 gaagatttcc aatcatttct aaaacatctc aacagtgagc atgcgctgga cgatagaagt
      721 acagcccaat gtagagtaca aatgcaggtt gtacagcagt tagagctaca gcttgcaaaa
      781 gacaaagaac gcctgcaagc catgatgacc cacctgcatg tgaagtctac agaacccaaa
      841 gccgcccctc agcccttgaa tctggtatca agtgtcactc tctccaagtc cgcatcggag
      901 gcttctccac agagcttacc tcatactcca acgaccccaa ccgcccccct gactcccgtc
      961 acccaaggcc cctctgtcat cacaaccacc agcatgcaca cggtgggacc catccgcagg
     1021 cggtactcag acaaatacaa cgtgcccatt tcgtcagcag atattgcgca gaaccaagaa
     1081 ttttataaga acgcagaagt tagaccacca tttacatatg catctttaat taggcaggcc
     1141 attctcgaat ctccagaaaa gcagctaaca ctaaatgaga tctataactg gttcacacga
     1201 atgtttgctt acttccgacg caacgcggcc acgtggaaga atgcagtgcg tcataatctt
     1261 agtcttcaca agtgttttgt gcgagtagaa aacgttaaag gggcagtatg gacagtggat
     1321 gaagtagaat tccaaaaacg aaggccacaa aagatcagtg gtaacccttc ccttattaaa
     1381 aacatgcaga gcagccacgc ctactgcaca cctctcaatg cagctttaca ggcttcaatg
     1441 gctgagaata gtatacctct atacactacc gcttccatgg gaaatcccac tctgggcaac
     1501 ttagccagcg caatacggga agagctgaac ggggcaatgg agcataccaa cagcaacgag
     1561 agtgacagca gtccaggcag atctcctatg caagccgtgc atcctgtaca cgtcaaagaa
     1621 gagcccctcg atccagagga agctgaaggg cccctgtcct tagtgacaac agccaaccac
     1681 agtccagatt ttgaccatga cagagattac gaagatgaac cagtaaacga ggacatggag
     1741 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1801 ggaaaggacg attggtccta tcttatgacg ttacg
//
